# First Complete Mitochondrial Genome Analysis of Tree Frog, Dryophytes flaviventris and Comparison with Dryophytes suweonensis

## TL;DR

This paper reports the first complete mitochondrial genome of Dryophytes flaviventris and compares it with D. suweonensis, finding no genetic differentiation between the two species.

## Contribution

The study provides the first complete mitogenome data for Dryophytes flaviventris and reveals that it is not genetically distinct from D. suweonensis.

## Key findings

- The mitogenomes of Dryophytes flaviventris and D. suweonensis are nearly identical in size and structure.
- Phylogenetic analysis shows that the two species do not form distinct clades, indicating no genetic differentiation.
- Mitochondrial DNA cannot differentiate between D. flaviventris and D. suweonensis, suggesting they are not reciprocally monophyletic.

## Abstract

Mitochondrial genomes (mitogenomes) play a key role in species identification and phylogenetic studies due to their stable gene arrangements and evolutionary insights. Dryophytes flaviventris, classified in 2020 and closely related to D. suweonensis, lacks mitochondrial DNA data for differentiation. This gap hinders accurate species identification, highlighting the need for further genomic studies. The complete mitogenome size of two D. flaviventris were 18,616–18,617 bp and those for two D. suweonensis were 18,610–18,616 bp, the mitogenomes of the two species consisting of 13 protein-coding genes (PCGs), two ribosomal RNA genes, 22 transfer RNA (tRNA) genes, and a D-loop. Phylogenetic analysis confirmed that the mitochondrial DNA of all four individuals formed a monophyletic group, showing no genetic differentiation. As a result, the two species do not form distinct clades, and mitogenomes could not differentiate them, suggesting they are not reciprocally monophyletic. This study presents the first mitogenome data for D. flaviventris and provides valuable insights into tree frog taxonomy.