# Novel Roles of Nestin in Postnatal Root Formation

## TL;DR

This study shows that Nestin is important for tooth root development and cementum formation in mice.

## Contribution

The study reveals novel roles of Nestin in postnatal root dentin and cementum development.

## Key findings

- Nestin knockout mice showed delayed molar eruption and reduced osteoclasts.
- KO mice had expanded predentin and abnormal dentin tubules.
- Reduced cellular cementum and key markers like DMP1 and OPN were observed in Nestin KO mice.

## Abstract

Background/Objectives: Nestin is an intermediate filament protein and a marker of odontoblasts, but its function in tooth and bone formation is largely unknown. This study aimed to determine whether Nestin plays a role in postnatal tooth formation. Methods: 4-week-old Nestin knockout (KO) mice were analyzed with a range of techniques, including X-ray imaging, uCT, backscattered and acid-etched casted SEM, FITC-confocal microscopy, H&E and TRAP staining, and immunohistochemistry. Results: The KO mice had no apparent difference in crown formation compared to age-matched wild-type (WT) but showed delayed molar eruption with reduced TRAP+ osteoclasts. More importantly, KO mice developed expanded predentin and shorter, thinner roots with irregular and shortened dentin tubules. Additionally, the Nestin KO mice exhibited a reduced cellular cementum mass with sharp reductions in DMP1, OPN, and BSP. Conclusions: These findings suggest that Nestin plays a critical role in the postnatal development of root dentin and cellular cementum.