# Effect of Vachellia tortilis Leaf Meal and Sunflower Oil Inclusion in Supplementary Diets of Lambs on In Vitro Short-Chain Fatty Acid and Gas Production and In Vivo Growth Performance

## TL;DR

This study explores the effects of adding Vachellia tortilis leaf meal and sunflower oil to lamb diets on digestion and growth, finding no negative impacts.

## Contribution

The study introduces Vachellia tortilis leaf meal as a potential protein supplement for lamb diets in subtropical regions.

## Key findings

- VT inclusion did not negatively affect lamb growth or in vitro gas production.
- No significant changes in short-chain fatty acids or methane production were observed.
- VT shows promise as a supplementary protein source but needs further validation with larger samples.

## Abstract

This study investigated the potential effects of Vachellia tortilis leaf meal (VT) and sunflower oil (SFO) supplementation on in vitro short-chain fatty acid (SCFA) production and gas production and the in vivo growth performance of lambs. The VT inclusion showed no statistically observed negative impact on growth performance or in vitro gas production, suggesting that VT might be a suitable supplementary protein source for lambs in the subtropical regions, given that further validation studies conform with the findings from the current study.

This study examined the effect of dietary Vachellia tortilis leaf meal and sunflower oil inclusion in supplementary diets on in vitro short-chain fatty acid (SCFA) and gas production and the in vivo growth performance of lambs. Four concentrate dietary treatments comprising control (CL), Vachellia tortilis leaf meal (VT), sunflower oil (SFO), and Vachellia tortilis leaf meal sunflower oil (VSFO) were formulated. Eight Merino lambs were blocked into two live weight blocks where animals within each block were randomly assigned to dietary treatments, making two animals per treatment. Lambs were offered a concentrate diet of 480 g/day per head, with urea-treated hay fed as a basal diet. Dietary effects were evaluated for in vitro short-chain fatty acid and gas production and 28-day growth performance of lambs over three study periods. The inclusion of VT and SFO showed no statistically significant effect on total SCFAs, acetate to propionate (A:P) ratio, methane (CH4), and carbon dioxide (CO2) production (p > 0.05), which might be attributable to low experimental units. The hourly A:P ratio and CH4 yield were the highest (p < 0.05) at 16 h of incubation. The inclusion of VT showed no statistically significant effect on growth performance and gas production due to the small sample size. Therefore, VT can be explored as a supplementary protein source in lamb diets given the impression of not showing any causative adverse effects on growth performance and in vitro gas production, although further research with larger sample sizes is needed to confirm these findings.