circTUBD1‐hnRNPK Regulates the Proliferation and Migration of LSCC by Targeting CCAR1

TL;DR

This study identifies a circular RNA called circTUBD1 that promotes laryngeal cancer growth and spread by interacting with a protein called hnRNPK and regulating a gene called CCAR1.

Contribution

The novel finding is that circTUBD1 forms a complex with hnRNPK and regulates CCAR1 to drive laryngeal squamous cell carcinoma progression.

Findings

circTUBD1 is significantly upregulated in LSCC and promotes cell proliferation, invasion, and migration.

circTUBD1 interacts with hnRNPK and regulates CCAR1 to facilitate LSCC progression.

Silencing circTUBD1 inhibits tumor growth and metastasis in mouse models.

Abstract

Laryngeal squamous cell carcinoma (LSCC) is one of the most prevalent malignancies of the head and neck region. Circular RNAs (circRNAs) have been found to exhibit abnormal expression patterns in various tumors and play pivotal roles in tumorigenesis and tumor progression.

Functional assays assessed proliferation, migration, and invasion. Mechanistic studies were performed to explore the interaction between circTUBD1 and heterogeneous nuclear ribonucleoprotein K (hnRNPK), as well as its regulation of Cell Cycle and Apoptosis Regulator 1 (CCAR1). In vivo experiments confirmed circTUBD1's role in tumor growth and metastasis.

We discovered that circTUBD1 is significantly upregulated in LSCC and promotes the proliferation, invasion, and migration of LSCC cells. circTUBD1 forms a circRNA‐protein complex with hnRNPK and facilitates LSCC progression by regulating CCAR1. Furthermore, in vivo experiments in mice demonstrated that silencing circTUBD1 inhibits the proliferation and metastasis of LSCC.

This study provides evidence that circTUBD1 is a potential tumor marker for LSCC and underscores the therapeutic potential of targeting circTUBD1 in this cancer type.

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