# CUsKit: a test to revolutionise the evaluation of the disinfection process

**Authors:** Débora Castro, Isabel Ferreri, Mariana Henriques, Isabel Carvalho

PMC · DOI: 10.1007/s00253-025-13456-8 · Applied Microbiology and Biotechnology · 2025-03-22

## TL;DR

CUsKit is a detection kit that helps track hydrogen peroxide disinfectants on surfaces, ensuring proper application and reducing microbial resistance.

## Contribution

CUsKit introduces a new method for detecting hydrogen peroxide disinfectants on surfaces up to 7 days post-application.

## Key findings

- CUsKit changes color from transparent to yellow-orange, indicating disinfectant presence.
- The color change correlates with antimicrobial efficacy on both porous and non-porous surfaces.
- The kit helps ensure correct disinfectant application, potentially reducing microbial resistance.

## Abstract

The overuse of surface disinfectants has been shown to lead to increased microbial resistance, often due to their incorrect application. The aim of this work was to develop and investigate the use of an indicative detection kit for hydrogen peroxide-based disinfectants on porous and non-porous surfaces. The detection kit allows for the detection of the disinfectant up to 7 days after its application, resulting in a colour change from transparent to yellow-orange on both on porous and non-porous surfaces. Concurrent antimicrobial tests were performed to establish the correlation between the colour change and the retention of the disinfectant on the surfaces, thereby confirming the maintenance of its antimicrobial efficacy.

Increased microbial resistance often results from overuse of disinfectants.

CUsKit is a hydrogen peroxide-based disinfectant detection kit.

CUsKit is an excellent option to prove the presence of disinfectants.

Increased microbial resistance often results from overuse of disinfectants.

CUsKit is a hydrogen peroxide-based disinfectant detection kit.

CUsKit is an excellent option to prove the presence of disinfectants.

## Linked entities

- **Chemicals:** hydrogen peroxide (PubChem CID 784)

## Full text

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## Figures

9 figures with captions in the complete paper: https://tomesphere.com/paper/PMC11929721/full.md

## References

9 references — full list in the complete paper: https://tomesphere.com/paper/PMC11929721/full.md

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Source: https://tomesphere.com/paper/PMC11929721