Preliminary feasibility study on DTI to assess the early brain injury in germinal matrix-intraventricular hemorrhage rats

TL;DR

This study shows that DTI can detect early brain changes in a rat model of germinal matrix-intraventricular hemorrhage, helping assess both short-term and long-term neurological effects.

Contribution

The study demonstrates the feasibility of using DTI to detect early microstructural brain injury in a GMH-IVH rat model.

Findings

DTI parameters showed changes in the striatum and other brain regions after GMH-IVH, correlating with behavioral abnormalities.

Transcriptome analysis revealed upregulated hemoglobin genes and downregulated neurodevelopment pathways 24 hours after GMH-IVH.

Long-term behavioral tests showed abnormal motor function in GMH-IVH rats compared to the sham group.

Abstract

To evaluate the feasibility and efficacy of diffusion tensor imaging (DTI) for detecting early brain microstructure alterations in germinal matrix-intraventricular hemorrhage (GMH-IVH) rat model. This study used a postnatal day 5 (PND 5) rat model of GMH-IVH. T2-weighted imaging and DTI were performed during acute (6 h and 24 h) and subacute (3d and 7d) phases after GMH-IVH. Four DTI parameters including fractional anisotropy (FA), mean diffusion (MD), axial diffusion (AD) and radial diffusion (RD) were collected in 9 specific brain regions to assess the brain microstructure alterations. Early and long-term neurological function tests were evaluated. Transcriptome sequencing analysis was also performed to investigate possible underlying mechanisms. Regional abnormalities after GMH-IVH were observed in T2-weighted images that showed significant hypointense in striatum region which close to the germinal matrix. DTI parameters also observed changes in striatum region in GMH-IVH. Alterations in other regions of brain including hippocampus, thalamus, external capsule and motor cortex also noted, which were associated with the abnormalities observed in behavioral experiments. Long-term behavioral tests show that compared to sham group, rats in GMH-IVH group caused abnormal motor function. In addition, at 24 h after GMH-IVH, transcriptome analysis results showed that the highly expressed differential genes encode hemoglobin components and down-regulate neurodevelopment-related pathways. DTI imaging allows the early assessment of neurological alteration in GMH-IVH rat pups, and providing great value in evaluating long-term behavioral deficits.

The online version contains supplementary material available at 10.1038/s41598-025-94934-x.

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