# Characterization of a cytokinin-binding protein locus in Mycobacterium tuberculosis

## TL;DR

This paper explores how Mycobacterium tuberculosis interacts with cytokinins, revealing a protein that binds these hormones and highlighting similarities with plant systems.

## Contribution

The study identifies a cytokinin-binding protein in M. tuberculosis and shows its unique role in copper sensitivity.

## Key findings

- Rv3719, a protein similar to plant cytokinin oxidases, does not restore resistance to nitric oxide or copper in proteasome-defective M. tuberculosis.
- Deletion of Rv3719 increases copper sensitivity in both wild-type and proteasome-defective M. tuberculosis strains.
- Rv3718c, a neighboring protein, binds cytokinins with high specificity.

## Abstract

Cytokinins are adenine-based hormones that have been well-characterized in plants but are also made by bacteria, including the human-exclusive pathogen Mycobacterium tuberculosis. Like plants, M. tuberculosis uses cytokinins to regulate gene expression. We previously established that cytokinin overaccumulation in M. tuberculosis results in a buildup of aldehydes produced during cytokinin breakdown. In plants, dedicated enzymes called cytokinin oxidases convert cytokinins into adenine and various aldehydes. Proteasome degradation-deficient M. tuberculosis, which cannot degrade the cytokinin-producing enzyme Log, accumulates several cytokinins and at least one cytokinin-associated aldehyde, resulting in increased sensitivity to nitric oxide and copper. We therefore hypothesized that M. tuberculosis encodes one or more cytokinin oxidases, and disruption of this enzyme might restore resistance to nitric oxide and copper in a proteasome-defective strain. Using a homology-based search, we identified Rv3719 as a protein with high similarity to a plant cytokinin oxidase. Deletion of this gene, however, did not restore nitric oxide or copper resistance to a degradation-defective mutant. Instead, we observed increased copper sensitivity when Rv3719 was deleted from either wild-type or proteasome-defective strains. Finally, we characterized Rv3718c, a protein encoded adjacent to Rv3719, and found that it bound a cytokinin with high specificity. Collectively, these data support a role for cytokinin activity in M. tuberculosis physiology that remains to be further elucidated.

Numerous bacterial species encode cytokinin-producing enzymes, the functions of which are almost completely unknown. This work contributes new knowledge to the cytokinin field for bacteria and reveals further conservation of cytokinin-associated proteins between plants and prokaryotes.