# Characterization and comparison of Schizosaccharomyces pombe cdc15 temperature-sensitive mutants

## TL;DR

This paper studies temperature-sensitive mutants of the Cdc15 protein in fission yeast to better understand its role in cell division.

## Contribution

A new cdc15 allele is identified that reveals Cdc15's role in recruiting the early secretory pathway to the division site.

## Key findings

- Multiple cdc15 temperature-sensitive mutants were characterized and compared.
- A new cdc15 allele was identified that affects secretory pathway recruitment.
- The mutants provide new tools for studying cytokinesis in S. pombe.

## Abstract

The F-BAR protein
Cdc15
is essential for cytokinesis in the fission yeast
Schizosaccharomyces pombe
, playing a key scaffolding role and connecting the actomyosin-based cytokinetic ring to the plasma membrane. Here, we compared

cdc15

temperature-sensitive mutants isolated in multiple genetic screens. We determined the mutations within each

cdc15

mutant allele and analyzed their growth at different temperatures. Additionally, we report a new

cdc15

allele that highlights the requirement for
Cdc15
in the recruitment of the early secretory pathway to the cellular division site. The new mutants described here expand the toolkit for studying cytokinesis in
S. pombe
.