# Automated sample tracking and parameter adaption for scanning laser optical tomography

**Authors:** Hannes Benecke, Firas Almadani, Johannes Heske, Tobias May, Ludger Overmeyer, Sonja Johannsmeier, Tammo Ripken

PMC · DOI: 10.1371/journal.pone.0318974 · PLOS One · 2025-03-19

## TL;DR

This paper introduces automated software techniques to improve the quality and consistency of scanning laser optical tomography imaging.

## Contribution

The novel contribution is the development of two algorithms for digital sample tracking and motion correction, enabling automated high-quality imaging.

## Key findings

- Digital sample tracking eliminates the need for manual alignment, improving imaging consistency.
- Motion artifacts are effectively corrected using a second algorithm, enhancing tomographic reconstruction.
- The combined algorithms increase system reliability and accessibility for a wide range of users.

## Abstract

Non-destructive, three-dimensional imaging techniques are of great importance in medicine as well as in technical analysis. In this context, it is of particular importance to generate reliable and repeatable results of high quality. This can be aided by automation of manual processes. One of these imaging techniques, the Scanning Laser Optical Tomography, currently requires manual sample alignment by the user to achieve the highest possible image quality. This alignment demands skillful hand-eye coordination as well as experience on the part of the user, and thus often leads to inconsistent imaging results. To overcome this problem, this paper presents a technique for software-based automation of this challenge. The sample is not physically aligned, but digitally detected and tracked during the acquisition. Residual motion artifacts that interfere with tomographic reconstruction are corrected using a second automation algorithm. The combination of the two new algorithms significantly improves the quality of imaging and also increases the reliability and degree of automation of the system, making it accessible to a wide range of users.

## Full-text entities

- **Diseases:** tremor (MESH:D014202), SLOT (MESH:D004401)
- **Chemicals:** agarose (MESH:D012685), benzyl alcohol (MESH:D019905), silicone oil (MESH:D012827), polymer (MESH:D011108), AP150Wacker (-), oil (MESH:D009821)
- **Species:** Homo sapiens (human, species) [taxon 9606]

## Full text

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## Figures

12 figures with captions in the complete paper: https://tomesphere.com/paper/PMC11922203/full.md

## References

28 references — full list in the complete paper: https://tomesphere.com/paper/PMC11922203/full.md

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Source: https://tomesphere.com/paper/PMC11922203