# The analysis of neutron reflectivity from Langmuir monolayers of lipids using molecular dynamics simulations: the role of lipid area

## TL;DR

This paper shows how the area per lipid in simulations affects the accuracy of neutron reflectometry data interpretation for lipid monolayers.

## Contribution

A protocol is proposed to combine experimental surface pressure with simulation-derived area per lipid for better model accuracy.

## Key findings

- Fit of number density profiles is highly sensitive to the assumed area per lipid.
- Correct simulation cell choice strongly influences downstream interpretation of experimental data.
- Combining experimental and simulation data improves model realism for lipid monolayers.

## Abstract

Biomolecular simulations are increasingly being used to generate detailed structural models to aid interpretation of neutron reflectometry (NR) data obtained from model biological membranes. Unlike globular systems, often studied by small-angle scattering, simulations of two-dimensional layers are sensitive to the simulation cell used which constrains the system laterally. We perform a careful analysis of NR data obtained from a monolayer of the lipid distearoylphosphatidylcholine at the air–water interface and show that the fit of number density profiles obtained from atomistic molecular dynamics simulation to the experimental data is very sensitive to the assumed area per lipid (APL). We propose a protocol for obtaining a realistic isotherm by combining the experimental surface pressure corresponding to a reflectometry measurement with an APL obtained from the simulation that best fits that data. Finally, we demonstrate how downstream interpretation of the experimental sample, derived from structural and dynamic properties of the atomistic model, depends strongly on the correct choice of simulation cell.