# Hepatic stereological analysis in obese Zucker rats (Leprfa) with dyslipidemia

## TL;DR

This study examines how obesity affects liver health in Zucker rats, finding that obese rats have significant liver changes linked to fatty liver disease.

## Contribution

The study provides new stereological evidence of hepatic alterations in obese Zucker rats, supporting their use as a model for obesity-related liver disease.

## Key findings

- Obese Zucker rats showed increased body and liver weight, cholesterol, and triacylglycerol levels compared to lean rats.
- Stereological analysis revealed lower hepatocyte volume and higher steatosis volume in obese rats.
- The findings suggest obesity significantly alters liver morphology and supports the use of this rat model for liver disease research.

## Abstract

To characterize histologically and stereologically the hepatic steatosis in obese Zucker (fat, n = 6, with a mutation in the leptin receptor – Leprfa) and control Zucker (lean, n = 6) rats, analyzing macroscopic and microscopic differences to understand the influence of obesity on hepatic pathology.

Zucker rats were fed standard chow for 90 days. Macroscopic, qualitative, and histoquantitative (stereological) approaches were used, involving body and liver weight measurement, morphological analysis, and histopathological classification of metabolic dysfunction-associated steatotic liver disease.

Zucker fat rats had higher body weight (p = 0.0022), liver weight (p = 0.0022), serum total cholesterol (p = 0.0022), and triacylglycerol (p = 0.0022) compared to Zucker lean rats. Stereological analysis showed that hepatocyte volume density (p = 0.0022) and total hepatocyte volume (p = 0.0001) were lower, and the volume density (p = 0.002) and total volume of steatosis (p = 0.002) were higher in Zucker fat rats compared to lean rats.

The findings indicated that obesity induces significant alterations in the hepatic morphology of Zucker rats, showing that hepatocyte volume is lower in obese animals. This study reinforces the utility of the obese Zucker rat model to investigate the effects of obesity on liver health and suggests hepatic steatosis requires therapeutic strategies focused on modulating these parameters.