Water Translocation and Photosynthetic Responses in Clones of Kentucky Bluegrass to Heterogeneous Water Supply

TL;DR

This study explores how Kentucky bluegrass clones share water and respond to drought stress, revealing differences in their physiological integration and photosynthesis.

Contribution

The study provides new insights into water translocation and photosynthetic responses in clonal plants under drought stress using isotope labeling.

Findings

Drought stress leads to water physiological integration among different clonal ramets of Kentucky bluegrass.

Proximal ramets are most affected by drought stress, while distal ramets are least affected.

Drought stress causes varying degrees of inhibition in photosynthetic-related indicators across clonal ramets.

Abstract

Drought stress is the most common threat to plant growth, while physiological integration can significantly enhance the drought tolerance of clonal plants, making it essential to research the behavior of clones under drought conditions and explore the potential applications of clonal plants. This study applied polyethylene-glycol-6000-induced stress to proximal, middle and distal clonal ramets of Kentucky bluegrass (Poa pratensis L.) and used an isotope labeling technique to evaluate the water physiological integration and photosynthetic capacity. When the proximal ramet was subjected to drought stress treatment, the decrease in 2H isotopes in the roots from 4 h to 6 h was significantly smaller than the increase in 2H isotopes in their own leaves. Additionally, the reductions in δ2H values of middle and distal ramets roots were 4.14 and 2.6 times greater, respectively, than the increases in their respective leaf δ2H values. The results indicate that under drought stress, water physiological integration was observed among different clonal ramets. In addition, drought stress inhibits the photosynthetic-related indicators in clonal ramets, with varying degrees of response and trends in photosynthetic characteristics among different clonal ramets. The proximal ramet treatment group, treated with polyethylene glycol 6000, was most affected by drought stress, while the distal ramet treatment group was least affected. The proximal ramet treatment group, treated with polyethylene glycol 6000, showed a decrease in water use efficiency after 6 h of drought treatment, while the other groups exhibited some increase. This indicates differences in water utilization and regulation among the different clonal ramets under drought stress. This study holds significant theoretical importance for exploring the characteristics of physiological integration and the photosynthetic mechanisms of Kentucky bluegrass clones under drought stress.

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