A miniature low-immunogenic platform for the biosynthesis of self-assembling protein nanoparticles
Almina I. Polinova, Anna V. Serkina, Marina V. Volkova, Aleksandr A. Gorbunov, Evgeniia P. Sannikova, Irek I. Gubaidullin, Aleksandr S. Komolov, Anna V. Rybakova, Marina Yu. Kopaeva, Konstantin S. Plokhikh, Georgy S. Peters, Artem A. Shatilov, Alexander A. Shtil
TL;DR
Researchers created a low-immunogenic platform for making self-assembling protein nanoparticles that could be used for vaccines or drug delivery.
Contribution
A new miniature platform using a 30-amino acid peptide replaces a previously used immunogenic protein.
Findings
The pepA1-based platform achieved 100% seroconversion rate in mice.
The platform showed low immunogenicity without the need for adjuvants.
Improved synthesis and purification procedures enhance its potential for vaccine development.
Abstract
Aims: Previously, to obtain antigen-presenting self-assembling protein nanoparticles (SAPN), we developed a biosynthetic platform combining the self-associating peptide L6KD and the SUMO protein. In the current work, the immunogenic SUMO was replaced with an artificial 30 amino acid long peptide pepA1. Methods: The immunogenic properties of the pepA1-SAPN were tested in mice using the pneumococcal PhtD19 and ovalbumin OVA257-280 antigens in the absence of adjuvants. Results and Conclusions: The updated SAPN showed a 100% seroconversion rate and low immunogenicity of the platform. Given the effective synthesis and improved purification procedure, the pepA1-based miniature platform looks promising for development of vaccines and vehicles for targeted delivery.
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Taxonomy
TopicsMonoclonal and Polyclonal Antibodies Research · Glycosylation and Glycoproteins Research · Bacteriophages and microbial interactions