Regulation of P suc , a sucrose-inducible promoter in Caulobacter crescentus

TL;DR

This paper studies how a specific promoter in Caulobacter crescentus responds to changes in sucrose levels.

Contribution

The study identifies P suc as a tunable and tightly regulated sucrose-inducible promoter in Caulobacter.

Findings

P suc is responsive to varying sucrose concentrations in the media.

The promoter remains active even when other carbon sources are present.

The promoter is tightly controlled and can be tuned for expression.

Abstract

In gram-negative bacteria, when nutrients are too large or too scarce to diffuse through outer membrane porins, TonB-dependent receptors (TBDRs) are utilized to actively translocate substrates across the outer membrane.
Caulobacter crescentus
is a gram-negative bacterium with a large set of TBDRs, many which have not been fully characterized. Previous studies identified SucA, a
Caulobacter
TBDR that transports sucrose. Our experiments further characterize the expression of
sucA
from the

P
suc

promoter and we identify

P
suc

as a tightly controlled, tunable promoter, responsive to changes in sucrose concentration with and without other carbon sources in the media.

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