The genome sequence of the Twenty-plume Moth, Alucita hexadactyla Linnaeus, 1758
Liam M. Crowley, Peter W. H. Holland, David C. Lees, Caroline Eve Mitchell, Toni De-Dios, Annabel Whibley, Daniel Berner, Jesper Boman
TL;DR
This paper presents the genome sequence of the Twenty-plume Moth, including a detailed assembly of its chromosomes and mitochondrial DNA.
Contribution
The study provides a high-quality, chromosome-level genome assembly for the Twenty-plume Moth.
Findings
The genome assembly is 878.53 megabases long, with 99.74% scaffolded into 30 chromosomal pseudomolecules.
The mitochondrial genome is 15.32 kilobases in length and has been fully assembled.
Abstract
We present a genome assembly from a specimen of Alucita hexadactyla (Twenty-plume Moth; Arthropoda; Insecta; Lepidoptera; Alucitidae). The genome sequence has a total length of 878.53 megabases. Most of the assembly (99.74%) is scaffolded into 30 chromosomal pseudomolecules, including the Z sex chromosome. The mitochondrial genome has also been assembled and is 15.32 kilobases in length.
Genes, proteins, chemicals, diseases, species, mutations and cell lines named across the full text — each resolved to its canonical identifier and authoritative record.
Click any figure to enlarge with its caption.
Figure 1
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Figure 5| Project information | |||
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| Alucita hexadactyla (twenty-plume moth) | ||
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| PRJEB73441 | ||
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| SAMEA112232562 | ||
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| 753150 | ||
| Specimen information | |||
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| ilAluHexa2 | SAMEA112233010 | whole organism |
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| ilAluHexa3 | SAMEA112233015 | whole organism |
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| ilAluHexa4 | SAMEA114806037 | whole organism |
| Sequencing information | |||
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| ERR12743807 | 9.09e+08 | 137.24 |
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| ERR12721083 | 2.62e+06 | 30.72 |
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| ERR13493920 | 8.48e+07 | 12.81 |
| Genome assembly | ||
|---|---|---|
| Assembly name | ilAluHexa2.1 | |
| Assembly accession | GCA_964059475.1 | |
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| Assembly level for primary assembly | chromosome | |
| Span (Mb) | 878.53 | |
| Number of contigs | 157 | |
| Number of scaffolds | 58 | |
| Longest scaffold (Mb) | 57.39 | |
| Assembly metrics | Measure |
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| Contig N50 length | 11.55 Mb |
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| Scaffold N50 length | 31.03 Mb |
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| Consensus quality (QV) | Primary: 63.5; alternate: 62.6;
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| Primary: 81.84%; alternate:
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| BUSCO
| C:98.1%[S:97.4%,D:0.7%],
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| Percentage of assembly mapped to
| 99.75% |
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| Sex chromosomes | Z |
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| Organelles | Mitochondrial genome: 15.32 kb |
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| INSDC accession | Name | Length (Mb) | GC% |
|---|---|---|---|
| 1 | 51.42 | 38 | |
| 2 | 36.8 | 38 | |
| 3 | 35.23 | 38 | |
| 4 | 34.24 | 38 | |
| 5 | 33.58 | 38 | |
| 6 | 33.37 | 38 | |
| 7 | 32.95 | 38 | |
| 8 | 32.92 | 38 | |
| 9 | 32.24 | 38 | |
| 10 | 31.39 | 38 | |
| 11 | 31.03 | 38 | |
| 12 | 30.97 | 38.5 | |
| 13 | 30.49 | 38 | |
| 14 | 29.24 | 38.5 | |
| 15 | 27.9 | 38.5 | |
| 16 | 27.57 | 38 | |
| 17 | 26.77 | 38 | |
| 18 | 26.76 | 38.5 | |
| 19 | 25.64 | 38.5 | |
| 20 | 25.03 | 38.5 | |
| 21 | 24.42 | 38.5 | |
| 22 | 24.2 | 38 | |
| 23 | 23.43 | 38.5 | |
| 24 | 19.75 | 39 | |
| 25 | 19.73 | 38.5 | |
| 26 | 19.72 | 39.5 | |
| 27 | 18.3 | 39 | |
| 28 | 18.04 | 38.5 | |
| 29 | 15.8 | 39 | |
| Z | 57.39 | 37.5 | |
| MT | 0.02 | 20.5 |
| Software tool | Version | Source |
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| BEDTools | 2.30.0 |
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| BLAST | 2.14.0 |
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| BlobToolKit | 4.3.9 |
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| BUSCO | 5.5.0 |
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| bwa-mem2 | 2.2.1 |
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| Cooler | 0.8.11 |
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| DIAMOND | 2.1.8 |
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| fasta_windows | 0.2.4 |
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| FastK | 427104ea91c78c3b8b8b49f1a7d6bbeaa869ba1c |
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| Gfastats | 1.3.6 |
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| GoaT CLI | 0.2.5 |
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| Hifiasm | 0.19.8-r603 |
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| HiGlass | 44086069ee7d4d3f6f3f0012569789ec138f42b84a
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| MerquryFK | d00d98157618f4e8d1a9190026b19b471055b22e |
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| Minimap2 | 2.24-r1122 |
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| MitoHiFi | 3 |
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| MultiQC | 1.14, 1.17, and 1.18 |
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| NCBI Datasets | 15.12.0 |
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| Nextflow | 23.10.0 |
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| PretextView | 0.2 |
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| purge_dups | None |
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| samtools | 1.19.2 |
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| sanger-tol/ascc | - |
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| sanger-tol/blobtoolkit | 0.5.1 |
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| Seqtk | 1.3 |
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| Singularity | 3.9.0 |
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| TreeVal | 1.2.0 |
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| YaHS | 1.2a.2 |
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- —Wellcome Trust
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Taxonomy
TopicsInsect Resistance and Genetics · Plant Virus Research Studies · Insect-Plant Interactions and Control
Species taxonomy
Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Endopterygota; Amphiesmenoptera; Lepidoptera; Glossata; Neolepidoptera; Heteroneura; Ditrysia; Obtectomera; Alucitoidea; Alucitidae; Alucita; Alucita hexadactyla Linnaeus, 1758 (NCBI:txid753150)
Background
Alucita hexadactyla is a common moth in the UK, belonging to the family Alucitidae and the group microlepidoptera ( Davis, 2012). Commonly called the Many-plumed or Twenty-plume moth, the wings of A. hexadactyla have an unusual morphology. Unlike a more typical moth with four distinct scaled wings, each wing of A. hexadactyla is made from six feather-like fronds, hence ‘hexadactyla’ meaning six-fingered in Greek. One study proposes that this may help the moth evade bats as the wing structure reduces the intensity of simulated ultrasonic bat calls ( Kovalev, 2016). The wings have a white, brown and black chevron pattern and have a 14–16 mm wingspan ( Butterfly Conservation, no date).
A. hexadactyla is the only member of the family in the UK and can be found widely across the UK and Ireland ( Manley, 2021). The species is commonly found in gardens and woodlands where honeysuckle ( Lonicera caprifolium, periclymenum, xylosteum) grows because the larvae feed on the buds, flowers, and leaves of the plant ( Ellis, 2021). The moths fly throughout the year and are drawn to light so are often seen at windows or lights around buildings ( Butterfly Conservation, no date; Wood, 1870).
The Alucitidae family is relatively small for a lepidopteran family, with roughly 180 species ( Watkins, 2005), but is still expanding. Various new species in the family have been discovered across the world in the past 20 years ( Byun, 2006; Landry & Landry, 2004; Ustjuzhanin & Kovtunovich, 2016), with a notable recent burst of discovery in Cameroon ( Ustjuzhanin et al., 2018; Ustjuzhanin et al., 2020). With recent discoveries of new species, and the potential for more, reference genomes for the family will be key to understanding the evolutionary history and phylogeny of Alucitidae and, more widely, lepidoptera. Here we present a chromosomally complete genome sequence for Alucita hexadactyla, based on a male specimen from Olton, West Midlands, United Kingdom ( Figure 1). This reference genome for Alucita hexadactyla is the first of the family and of the Alucitoidea superfamily.
Photograph of the Alucita hexadactyla (ilAluHexa2) specimen used for genome sequencing.
Genome sequence report
Sequencing data
The genome of a specimen of Alucita hexadactyla ( Figure 1) was sequenced using Pacific Biosciences single-molecule HiFi long reads, generating 30.72 Gb from 2.62 million reads. GenomeScope analysis of the PacBio HiFi data estimated the haploid genome size at 871.06 Mb, with a heterozygosity of 0.79% and repeat content of 44.71%. These values provide an initial assessment of genome complexity and the challenges anticipated during assembly. Based on this estimated genome size, the sequencing data provided approximately 34.0x coverage of the genome. Chromosome conformation Hi-C data produced 137.24 Gb from 908.89 million reads. Table 1 summarises the specimen and sequencing information, including the BioProject, study name, BioSample numbers, and sequencing data for each technology.
Table 1.: Specimen and sequencing data for Alucita hexadactyla.
Assembly statistics
The primary haplotype was assembled, and contigs corresponding to an alternate haplotype were also deposited in INSDC databases. The assembly was improved by manual curation, which corrected 14 misjoins or missing joins and removed three haplotypic duplications. The final assembly has a total length of 878.53 Mb in 58 scaffolds, with 99 gaps, and a scaffold N50 of 31.03 Mb ( Table 2).
Table 2.: Genome assembly data for Alucita hexadactyla.
The snail plot in Figure 2 provides a summary of the assembly statistics, indicating the distribution of scaffold lengths and other assembly metrics. Figure 3 shows the distribution of scaffolds by GC proportion and coverage. Figure 4 presents a cumulative assembly plot, with separate curves representing different scaffold subsets assigned to various phyla, illustrating the completeness of the assembly.
Genome assembly of Alucita hexadactyla, : metrics.The BlobToolKit snail plot provides an overview of assembly metrics and BUSCO gene completeness. The circumference represents the length of the whole genome sequence, and the main plot is divided into 1,000 bins around the circumference. The outermost blue tracks display the distribution of GC, AT, and N percentages across the bins. Scaffolds are arranged clockwise from longest to shortest and are depicted in dark grey. The longest scaffold is indicated by the red arc, and the deeper orange and pale orange arcs represent the N50 and N90 lengths. A light grey spiral at the centre shows the cumulative scaffold count on a logarithmic scale. A summary of complete, fragmented, duplicated, and missing BUSCO genes in the lepidoptera_odb10 set is presented at the top right. An interactive version of this figure is available at https://blobtoolkit.genomehubs.org/view/GCA_964059475.1/dataset/GCA_964059475.1/snail.
Genome assembly of Alucita hexadactyla, : BlobToolKit GC-coverage plot.Blob plot showing sequence coverage (vertical axis) and GC content (horizontal axis). The circles represent scaffolds, with the size proportional to scaffold length and the colour representing phylum membership. The histograms along the axes display the total length of sequences distributed across different levels of coverage and GC content. An interactive version of this figure is available at https://blobtoolkit.genomehubs.org/view/GCA_964059475.1/blob.
Genome assembly of Alucita hexadactyla : BlobToolKit cumulative sequence plot.The grey line shows cumulative length for all scaffolds. Coloured lines show cumulative lengths of scaffolds assigned to each phylum using the buscogenes taxrule. An interactive version of this figure is available at https://blobtoolkit.genomehubs.org/view/GCA_964059475.1/dataset/GCA_964059475.1/cumulative.
Most of the assembly sequence (99.75%) was assigned to 30 chromosomal-level scaffolds, representing 29 autosomes and the Z sex chromosome. These chromosome-level scaffolds, confirmed by Hi-C data, are named according to size ( Figure 5; Table 3). During curation, chromosome Z was identified based on the Hi-C signal.
Genome assembly of Alucita hexadactyla : Hi-C contact map of the assembly, visualised using HiGlass.Chromosomes are shown in order of size from left to right and top to bottom. An interactive version of this figure may be viewed at https://genome-note-higlass.tol.sanger.ac.uk/l/?d=IJtVqGMSTHuQ0wxO9542LA.
Table 3.: Chromosomal pseudomolecules in the genome assembly of Alucita hexadactyla, ilAluHexa2.
The mitochondrial genome was also assembled. This sequence is included as a contig in the multifasta file of the genome submission and as a standalone record in GenBank.
Assembly quality metrics
The estimated Quality Value (QV) and k-mer completeness metrics, along with BUSCO completeness scores, were calculated for each haplotype and the combined assembly. The QV reflects the base-level accuracy of the assembly, while k-mer completeness indicates the proportion of expected k-mers identified in the assembly. BUSCO scores provide a measure of completeness based on benchmarking universal single-copy orthologues.
The primary haplotype has a QV of 63.5, and the combined primary and alternate assemblies achieve an estimated QV of 63.0. The k-mer completeness for the primary haplotype is 81.84%, and for the alternate haplotype it is 79.84%. The combined primary and alternate assemblies achieve a k-mer completeness of 99.02%. BUSCO analysis using the lepidoptera_odb10 reference set ( n = 5,286) indicated a completeness score of 98.1% (single = 97.4%, duplicated = 0.7%).
Table 2 provides assembly metric benchmarks adapted from Rhie et al. (2021) and the Earth BioGenome Project Report on Assembly Standards September 2024. The assembly achieves the EBP reference standard of 6.C.63.
Methods
Sample acquisition and DNA barcoding
An adult male Alucita hexadactyla (specimen ID Ox002335, ToLID ilAluHexa2) was collected from Olton, West Midlands, United Kingdom (latitude 52.44, longitude –1.81) on 2022-07-16 by potting. The specimen was collected and identified by Liam Crowley (University of Oxford) and preserved on dry ice. This specimen was used for genome sequencing.
The specimen used for Hi-C sequencing (specimen ID Ox002338, ToLID ilAluHexa3) was a adult specimen collected from Wallingford, Oxfordshire, United Kingdom (latitude 51.6, longitude –1.14) on 2022-07-11, using a light trap. The specimen was collected and identified by Peter Holland (University of Oxford) and preserved on dry ice.
The specimen used for RNA sequencing (specimen ID NHMUK014584894, ToLID ilAluHexa4) was collected from Lucas Road, High Wycombe, England, United Kingdom (latitude 51.63, longitude –0.74) on 2022-07-08. The specimen was collected and identified by David Lees (Natural History Museum) and preserved by dry freezing (–80 °C).
The initial identification was verified by an additional DNA barcoding process according to the framework developed by Twyford et al. (2024). A small sample was dissected from the specimen and stored in ethanol, while the remaining parts were shipped on dry ice to the Wellcome Sanger Institute (WSI) ( Pereira et al., 2022). The tissue was lysed, the COI marker region was amplified by PCR, and amplicons were sequenced and compared to the BOLD database, confirming the species identification ( Crowley et al., 2023). Following whole genome sequence generation, the relevant DNA barcode region was also used alongside the initial barcoding data for sample tracking at the WSI ( Twyford et al., 2024). The standard operating procedures for Darwin Tree of Life barcoding have been deposited on protocols.io ( Beasley et al., 2023).
Metadata collection for samples adhered to the Darwin Tree of Life project standards described by Lawniczak et al. (2022).
Nucleic acid extraction
The workflow for high molecular weight (HMW) DNA extraction at the Wellcome Sanger Institute (WSI) Tree of Life Core Laboratory includes a sequence of procedures: sample preparation and homogenisation, DNA extraction, fragmentation and purification. Detailed protocols are available on protocols.io ( Denton et al., 2023b).
The ilAluHexa2 sample was prepared for DNA extraction by weighing and dissecting it on dry ice ( Jay et al., 2023). Tissue from the whole organism was homogenised using a PowerMasher II tissue disruptor ( Denton et al., 2023a). HMW DNA was extracted in the WSI Scientific Operations core using the Automated MagAttract v2 protocol ( Oatley et al., 2023). The DNA was sheared into an average fragment size of 12–20 kb in a Megaruptor 3 system ( Bates et al., 2023). Sheared DNA was purified by solid-phase reversible immobilisation, using AMPure PB beads to eliminate shorter fragments and concentrate the DNA ( Strickland et al., 2023). The concentration of the sheared and purified DNA was assessed using a Nanodrop spectrophotometer and Qubit Fluorometer using the Qubit dsDNA High Sensitivity Assay kit. Fragment size distribution was evaluated by running the sample on the FemtoPulse system.
RNA was extracted from whole organism tissue of ilAluHexa4 in the Tree of Life Laboratory at the WSI using the RNA Extraction: Automated MagMax™ mirVana protocol ( do Amaral et al., 2023). The RNA concentration was assessed using a Nanodrop spectrophotometer and a Qubit Fluorometer using the Qubit RNA Broad-Range Assay kit. Analysis of the integrity of the RNA was done using the Agilent RNA 6000 Pico Kit and Eukaryotic Total RNA assay.
Hi-C sample preparation
Tissue from the whole organism of the ilAluHexa3 sample was processed for Hi-C sequencing at the WSI Scientific Operations core, using the Arima-HiC v2 kit. In brief, 20–50 mg of frozen tissue (stored at –80 °C) was fixed, and the DNA crosslinked using a TC buffer with 22% formaldehyde concentration. After crosslinking, the tissue was homogenised using the Diagnocine Power Masher-II and BioMasher-II tubes and pestles. Following the Arima-HiC v2 kit manufacturer's instructions, crosslinked DNA was digested using a restriction enzyme master mix. The 5’-overhangs were filled in and labelled with biotinylated nucleotides and proximally ligated. An overnight incubation was carried out for enzymes to digest remaining proteins and for crosslinks to reverse. A clean up was performed with SPRIselect beads prior to library preparation. Additionally, the biotinylation percentage was estimated using the Qubit Fluorometer v4.0 (Thermo Fisher Scientific) and Qubit HS Assay Kit and Arima-HiC v2 QC beads.
Library preparation and sequencing
Library preparation and sequencing were performed at the WSI Scientific Operations core.
** PacBio HiFi **
At a minimum, samples were required to have an average fragment size exceeding 8 kb and a total mass over 400 ng to proceed to the low input SMRTbell Prep Kit 3.0 protocol (Pacific Biosciences, California, USA), depending on genome size and sequencing depth required. Libraries were prepared using the SMRTbell Prep Kit 3.0 (Pacific Biosciences, California, USA) as per the manufacturer's instructions. The kit includes the reagents required for end repair/A-tailing, adapter ligation, post-ligation SMRTbell bead cleanup, and nuclease treatment. Following the manufacturer’s instructions, size selection and clean up was carried out using diluted AMPure PB beads (Pacific Biosciences, California, USA). DNA concentration was quantified using the Qubit Fluorometer v4.0 (Thermo Fisher Scientific) with Qubit 1X dsDNA HS assay kit and the final library fragment size analysis was carried out using the Agilent Femto Pulse Automated Pulsed Field CE Instrument (Agilent Technologies) and gDNA 55kb BAC analysis kit.
Samples were sequenced using the Sequel IIe system (Pacific Biosciences, California, USA). The concentration of the library loaded onto the Sequel IIe was in the range 40–135 pM. The SMRT link software, a PacBio web-based end-to-end workflow manager, was used to set-up and monitor the run, as well as perform primary and secondary analysis of the data upon completion.
** Hi-C **
For Hi-C library preparation, DNA was fragmented using the Covaris E220 sonicator (Covaris) and size selected using SPRISelect beads to 400 to 600 bp. The DNA was then enriched using the Arima-HiC v2 kit Enrichment beads. Using the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs) for end repair, a-tailing, and adapter ligation. This uses a custom protocol which resembles the standard NEBNext Ultra II DNA Library Prep protocol but where library preparation occurs while DNA is bound to the Enrichment beads. For library amplification, 10 to 16 PCR cycles were required, determined by the sample biotinylation percentage. The Hi-C sequencing was performed using paired-end sequencing with a read length of 150 bp on an Illumina NovaSeq X instrument.
** RNA **
Poly(A) RNA-Seq libraries were constructed using the NEB Ultra II RNA Library Prep kit, following the manufacturer’s instructions. RNA sequencing was performed on the Illumina NovaSeq X instrument.
Genome assembly, curation and evaluation
** Assembly **
Prior to assembly of the PacBio HiFi reads, a database of k-mer counts ( k = 31) was generated from the filtered reads using FastK. GenomeScope2 ( Ranallo-Benavidez et al., 2020) was used to analyse the k-mer frequency distributions, providing estimates of genome size, heterozygosity, and repeat content.
The HiFi reads were first assembled using Hifiasm ( Cheng et al., 2021) with the --primary option. Haplotypic duplications were identified and removed using purge_dups ( Guan et al., 2020). The Hi-C reads were mapped to the primary contigs using bwa-mem2 ( Vasimuddin et al., 2019). The contigs were further scaffolded using the provided Hi-C data ( Rao et al., 2014) in YaHS ( Zhou et al., 2023) using the --break option for handling potential misassemblies. The scaffolded assemblies were evaluated using Gfastats ( Formenti et al., 2022), BUSCO ( Manni et al., 2021) and MERQURY.FK ( Rhie et al., 2020).
The mitochondrial genome was assembled using MitoHiFi ( Uliano-Silva et al., 2023), which runs MitoFinder ( Allio et al., 2020) and uses these annotations to select the final mitochondrial contig and to ensure the general quality of the sequence.
** Assembly curation **
The assembly was decontaminated using the Assembly Screen for Cobionts and Contaminants (ASCC) pipeline (article in preparation). Flat files and maps used in curation were generated in TreeVal ( Pointon et al., 2023). Manual curation was primarily conducted using PretextView ( Harry, 2022), with additional insights provided by JBrowse2 ( Diesh et al., 2023) and HiGlass ( Kerpedjiev et al., 2018). Scaffolds were visually inspected and corrected as described by Howe et al. (2021). Any identified contamination, missed joins, and mis-joins were corrected, and duplicate sequences were tagged and removed. Sex chromosomes were identified by Hi-C coverage analysis. The curation process is documented at https://gitlab.com/wtsi-grit/rapid-curation (article in preparation).
** Assembly quality assessment **
The Merqury.FK tool ( Rhie et al., 2020), run in a Singularity container ( Kurtzer et al., 2017), was used to evaluate k-mer completeness and assembly quality for the primary and alternate haplotypes using the k-mer databases ( k = 31) that were computed prior to genome assembly. The analysis outputs included assembly QV scores and completeness statistics.
A Hi-C contact map was produced for the final version of the assembly. The Hi-C reads were aligned using bwa-mem2 ( Vasimuddin et al., 2019) and the alignment files were combined using SAMtools ( Danecek et al., 2021). The Hi-C alignments were converted into a contact map using BEDTools ( Quinlan & Hall, 2010) and the Cooler tool suite ( Abdennur & Mirny, 2020). The contact map is visualised in HiGlass ( Kerpedjiev et al., 2018).
The blobtoolkit pipeline is a Nextflow port of the previous Snakemake Blobtoolkit pipeline ( Challis et al., 2020). It aligns the PacBio reads in SAMtools and minimap2 ( Li, 2018) and generates coverage tracks for regions of fixed size. In parallel, it queries the GoaT database ( Challis et al., 2023) to identify all matching BUSCO lineages to run BUSCO ( Manni et al., 2021). For the three domain-level BUSCO lineages, the pipeline aligns the BUSCO genes to the UniProt Reference Proteomes database ( Bateman et al., 2023) with DIAMOND blastp ( Buchfink et al., 2021). The genome is also divided into chunks according to the density of the BUSCO genes from the closest taxonomic lineage, and each chunk is aligned to the UniProt Reference Proteomes database using DIAMOND blastx. Genome sequences without a hit are chunked using seqtk and aligned to the NT database with blastn ( Altschul et al., 1990). The blobtools suite combines all these outputs into a blobdir for visualisation.
The blobtoolkit pipeline was developed using nf-core tooling ( Ewels et al., 2020) and MultiQC ( Ewels et al., 2016), relying on the Conda package manager, the Bioconda initiative ( Grüning et al., 2018), the Biocontainers infrastructure ( da Veiga Leprevost et al., 2017), as well as the Docker ( Merkel, 2014) and Singularity ( Kurtzer et al., 2017) containerisation solutions.
Table 4 contains a list of relevant software tool versions and sources.
Wellcome Sanger Institute – Legal and Governance
The materials that have contributed to this genome note have been supplied by a Darwin Tree of Life Partner. The submission of materials by a Darwin Tree of Life Partner is subject to the ‘Darwin Tree of Life Project Sampling Code of Practice’, which can be found in full on the Darwin Tree of Life website here. By agreeing with and signing up to the Sampling Code of Practice, the Darwin Tree of Life Partner agrees they will meet the legal and ethical requirements and standards set out within this document in respect of all samples acquired for, and supplied to, the Darwin Tree of Life Project.
Further, the Wellcome Sanger Institute employs a process whereby due diligence is carried out proportionate to the nature of the materials themselves, and the circumstances under which they have been/are to be collected and provided for use. The purpose of this is to address and mitigate any potential legal and/or ethical implications of receipt and use of the materials as part of the research project, and to ensure that in doing so we align with best practice wherever possible. The overarching areas of consideration are:
• Ethical review of provenance and sourcing of the material
• Legality of collection, transfer and use (national and international)
Each transfer of samples is further undertaken according to a Research Collaboration Agreement or Material Transfer Agreement entered into by the Darwin Tree of Life Partner, Genome Research Limited (operating as the Wellcome Sanger Institute), and in some circumstances other Darwin Tree of Life collaborators.
The reference list from the paper itself. Each links out to its DOI / PubMed record.
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- 8Butterfly Conservation: Twenty-plume moth. [no date]. Reference Source