# Anthelmintic Potential of Conjugated Long-Chain Fatty Acids Isolated from the Bioluminescent Mushroom Neonothopanus gardneri

## TL;DR

This study identifies new fatty acids from a bioluminescent mushroom that show promise as antiparasitic agents for schistosomiasis.

## Contribution

The discovery of new conjugated long-chain fatty acids with anthelmintic activity from Neonothopanus gardneri.

## Key findings

- Compound 3 showed significant antiparasitic activity against Schistosoma mansoni with an EC50 < 10 μM.
- No toxicity was observed in mammalian cells or C. elegans for the tested compounds.

## Abstract

With praziquantel being the sole available drug for schistosomiasis,
identifying novel anthelmintic agents is imperative. A chemical investigation
of the fruiting body of the bioluminescent mushroom Neonothopanus
gardneri Berk. resulted in the isolation of new conjugated
long-chain fatty acids (8E,10E,12S,13S)-12,13-dihydroxy-7-oxo-octadeca-8,10-dienoic
acid (1) and (7S,8S,9E,11E)-7,8-dihydroxy-13-oxo-octadeca-9,11-dienoic
acid (2) and three previously described compounds, (7R,8R,9Z)-7,8-dihydroxyoctadec-9-enoic
acid (3), (2E)-dec-2-ene-1,10-dioic
acid (4), and a ketolactone marasmeno-1,15-dione (5). Their planar structures were elucidated based on 1D and
2D NMR and MS/MS spectroscopic analyses. Compound 3 displayed
significant antiparasitic activity against Schistosoma mansoni
ex vivo (EC50 < 10 μM). No toxicity was
observed in mammalian cells or Caenorhabditis elegans.