Cloning of the DlERF10 gene from Diospyros lotus L. and cold tolerance analysis of the DlERF10 gene in transgenic tobacco plants
Ruijin Zhou, Shuda Li, Xiaona Zhang, Yingying Wang, Huiling Hu, Diaa Abd El-Moneim, Diaa Abd El-Moneim, Diaa Abd El-Moneim
TL;DR
Researchers cloned the DlERF10 gene from a cold-tolerant persimmon species and found it improves cold resistance in tobacco plants.
Contribution
The DlERF10 gene from Diospyros lotus is identified as a novel candidate for enhancing cold tolerance in plants.
Findings
Transgenic tobacco plants overexpressing DlERF10 showed less damage under low-temperature stress.
DlERF10 increased protective enzyme activity and reduced membrane damage in tobacco leaves.
The gene is proposed as a candidate for improving cold tolerance in Diospyros species.
Abstract
In the north of China, Diospyros plants are vulnerable to low-temperature damage in winter and is considered as a major factor restricting the development of the persimmon industry in Northern China. Diospyros lotus L. is featured by high survival potential of seedlings, cold tolerance, and grafting affinity with D. kaki Thunb. D. lotus has been frequently used as rootstocks for Diospyros spp. ERF transcriptional factors are a subfamily of the AP2/ERF gene family and play an important role in plant growth and stress tolerance. To explore the structure and function of the ERF transcription factors in D. lotus, we performed RT-PCR to clone DlERF10 from the leaves. The DlERF10 gene was 1104 bp long, encoding 367 amino acids. In order to deeply study the cold tolerance of DlERF10 gene, the pBI121-DlERF10 overexpression vector was constructed, and agrobacterium-mediated transformation was…
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Taxonomy
TopicsPlant Gene Expression Analysis · Light effects on plants · Biochemical and biochemical processes