Characterization and comparison of temperature-sensitive Schizosaccharomyces pombe mutants of the septation initiation network scaffolds, Cdc11 and Sid4

TL;DR

This paper studies temperature-sensitive mutants of two proteins in a yeast cell division network to better understand how they function.

Contribution

The study characterizes new temperature-sensitive mutants of Cdc11 and Sid4 in S. pombe, expanding tools for SIN research.

Findings

Temperature-sensitive cdc11 and sid4 mutants were identified and characterized.

Mutant alleles showed distinct phenotypes and growth patterns compared to previously known mutants.

The findings enhance understanding of SIN scaffold assembly at spindle pole bodies.

Abstract

The
Schizosaccharomyces pombe
septation initiation network (SIN) is required for cytokinesis and septation. The SIN includes a protein kinase cascade that is assembled at spindle pole bodies (SPBs) in a cell cycle specific manner on a scaffold consisting of
Cdc11
, related to human centriolin, and the a-helical protein
Sid4
. Here, we characterized temperature-sensitive

cdc11

and

sid4

mutants isolated in the 1990s. We determined the mutations within each allele, examined their phenotypes, and analyzed their growth compared with previously characterized mutant alleles. The new mutants described here expand the toolkit for studying how the SIN assembles at SPBs.

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