# sqt-3(sc63) is an alternative CRISPR/Cas9 co-conversion marker in Caenorhabditis elegans

## TL;DR

This paper introduces a new genetic marker for tracking genome edits in C. elegans using CRISPR/Cas9.

## Contribution

The study introduces sqt-3(sc63) as a novel co-conversion marker on chromosome V for CRISPR/Cas9 genome editing in C. elegans.

## Key findings

- sqt-3(sc63) is a functional co-conversion marker for CRISPR/Cas9 genome editing in C. elegans.
- Its location on chromosome V makes it useful for editing targets on chromosome II.
- The conserved nature of sqt-3 suggests potential use in other nematode species.

## Abstract

The identification of genome-edited individuals by CRISPR/Cas9 in

Caenorhabditis elegans

often relies on the introduction of a second mutation with a visible phenotype. Popular targets for such co-conversion are

dpy-10

and

sqt-1

, both located on chromosome II. In this study, we introduce

sqt-3
(
sc63
)

V
as an alternative CRISPR/Cas9 co-conversion marker to facilitate the generation and detection of genome edits in

C. elegans

. Its location on chromosome V makes

sqt-3

a desirable alternative to

dpy-10

and

sqt-1

when editing targets on chromosome II. Additionally, the conserved nature of

sqt-3

provides a potential applicability in other nematode species.