A first trial to supplement local hardwood sawdust at the first soaking for the cultivation of Shiitake (Lentinula edodes)
Stephanie Nabhan, Soukayna Haidar Ahmad, Zeina El Sebaaly, Teodor Nedelin, Youssef Najib Sassine

TL;DR
This study explores using nano-sized amino acid supplements to improve shiitake mushroom cultivation on local hardwood sawdust, finding some benefits in yield and quality.
Contribution
The first investigation of nano-supplementation with amino acids in shiitake mushroom cultivation on local hardwood substrates.
Findings
Nano-supplementation improved biological yield by up to 22.5% in supplemented treatments.
Supplemented maple sawdust showed faster fruiting and comparable yield to oak sawdust.
Mushroom quality improved with higher protein and fiber content in supplemented substrates.
Abstract
Supplementation of the growing substrate has been reported to enhance the production of cultivated mushrooms; however, supplementation using nano-sized additives is not yet investigated on Shiitake (Lentinula edodes). The study investigated the potential of a nano-supplement (Lithovit®-Amino25) containing an admixture of 25% L-amino acids on shiitake cultivated on sawdust from locally available oak, maple, and apple trees (oak sawdust: OS, maple sawdust: MS, and apple sawdust: AS). Initially, sawdusts were enriched equally with wheat bran (WB) and at the first soaking, Lithovit®-Amino25 was applied at 5 g/L. Treatments were: T1: OS-WB (control), T2: OS-WB+ nano-amino, T3: MS-WB, T4: MS-WB+ nano-amino, T5: AS-WB, and T6: AS-WB+ nano-amino. Among non-supplemented substrates, complete mycelia run, fruiting, and harvest dates were faster in T1 than in T3 and T5. Complete mycelial…
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Taxonomy
TopicsFungal Biology and Applications · Mycorrhizal Fungi and Plant Interactions · Polysaccharides and Plant Cell Walls
