# Wild Birds’ Genetic Resources Bank: Feather Follicle Cell Culture as a Possible Source of Stem Cells

## TL;DR

This paper explores using feather follicle cells from wild birds as a practical source for stem cells and genetic conservation.

## Contribution

A new method for culturing feather follicle cells from wild birds is proposed for biobanking and genetic research.

## Key findings

- Feather follicle cells can be successfully cultured using a specific protocol involving collagenase digestion and DMEM medium.
- The method is effective when samples are collected within 6 hours after the bird's death.
- The cells show strong proliferation and form colonies suitable for biobank applications.

## Abstract

Follicular cells represent a valuable resource for genetic research, biotechnology and cryopreservation in biobanks, particularly for the conservation of endangered species. They offer a more practical alternative to gametes, embryos and fibroblasts. Collection of these cells can be achieved through feather plucking. Feather samples were opened with a scalpel and the feather pulp was washed with PBS, cut into cubes and digested in collagenase type IV. Cultivation was carried out in DMEM culture medium with 15% fetal bovine serum, 1% penicillin/streptomycin and 0.5% amphotericin, under incubation conditions of 39.5 °C and 5% CO2. Passages were carried out with 5% EDTA for 5 min. The culture was successful, with great cell proliferation, adherence to plastic and aggregation into cell colonies. This method was effective in obtaining feather follicle cells from wild birds, especially when collected up to 6 h after their death, and can serve as a base protocol for research with feather follicle cells aiming to create biobanks.