# Effect of yogurt probiotic liquid and powder supplementation on hematology and biochemistry blood levels of layer-phase laying hens

## TL;DR

This study found that 4% probiotic powder is most effective in reducing harmful blood markers in laying hens compared to other probiotic forms.

## Contribution

The study identifies 4% probiotic powder as the optimal form for improving blood health in laying hens.

## Key findings

- 4% probiotic powder reduced neutrophil, lymphocyte, and N/L ratio by 53.96%, 27.84%, and 36.25%, respectively.
- Alkaline phosphatase and cholesterol levels were reduced by 53.6% and 42.4% with 4% probiotic powder.
- Probiotic supplementation improved all measured blood parameters compared to the control group.

## Abstract

The aim of this study was to determine the best form and concentration of yogurt probiotics that can reduce leucocyte, neutrophil, lymphocyte, alkaline phosphatase, and cholesterol levels to normal limits.

There were 35 Isa Brown laying hens aged 40 weeks with health conditions chosen as the object of research. We used a completely randomized design method with seven treatments. Treatments included P0: basal feed; T1: basal feed and probiotic liquid 2%; T2: basal feed and probiotic liquid 3%; T3: basal feed and probiotic liquid 4%; T4: basal feed and probiotic powder 2%; T5: basal feed and probiotic powder 3%; and T6: basal feed and probiotic powder 4%. On day 35 of the research, we collected blood samples. We analyzed the data using analysis of variance, followed by Duncan’s multiple range test.

This showed that probiotic liquid and powdered yogurt had a significant effect (p < 0.05) on all parameters. Supplementation with 4% probiotic powder reduced neutrophil (53.96%), lymphocyte (27.84%), and N/L ratios (36.25%); alkaline phosphatase levels (53.6%); and cholesterol levels (ApB 26.65% and LDL 42.4%) compared to the control.

This study shows that all probiotic supplementation shows improvement in the studied parameters, but the addition of 4% probiotic powder had the best result for reducing neutrophil, lymphocyte, N/L ratio, alkaline phosphatase, and cholesterol levels compared to the control and probiotic liquid.