Genome-Wide Identification and Expression Analyses of Glycoside Hydrolase Family 18 Genes During Nodule Symbiosis in Glycine max
Rujie Li, Chuanjie Gou, Ke Zhang, Milan He, Lanxin Li, Fanjiang Kong, Zhihui Sun, Huan Liu
TL;DR
This study identifies and analyzes 36 GH18 genes in soybean, focusing on their role during nodule symbiosis and their response to environmental and developmental factors.
Contribution
The paper provides a comprehensive genome-wide analysis of GH18 genes in soybean, highlighting their classification, evolution, and nodulation-specific expression.
Findings
36 GH18 genes were identified in soybean, distributed across 16 chromosomes and grouped into five subclades.
Class III and V chitinases are the major subclades, with distinct amino acid and molecular weight ranges.
Three GH18 genes (GmGH18-15, GmGH18-24, GmGH18-33) showed nodulation-specific expression confirmed by RT-qPCR.
Abstract
Glycoside hydrolase family 18 (GH18) proteins can hydrolyze the β-1,4-glycosidic bonds of chitin, which is a common structure component of insect exoskeletons and fungal cell walls. In this study, 36 GH18 genes were identified and subjected to bioinformatic analysis based on the genomic data of Glycine max. They were distributed in 16 out of 20 tested soybean chromosomes. According to the amino acid sequences, they can be further divided into five subclades. Class III chitinases (22 members) and class V chitinases (6 members) are the major two subclades. The amino acid size of soybean GH18 proteins ranges from 173 amino acids (aa) to 820 aa and the molecular weight ranges from 19.46 kDa to 91.01 kDa. From an evolutionary perspective, soybean GH18 genes are closely related to Medicago (17 collinear loci with soybean) and Lotus (23 collinear loci with soybean). Promoter analysis revealed…
Genes, proteins, chemicals, diseases, species, mutations and cell lines named across the full text — each resolved to its canonical identifier and authoritative record.
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Taxonomy
TopicsLegume Nitrogen Fixing Symbiosis · Studies on Chitinases and Chitosanases · Nematode management and characterization studies