# ID3 promotes erythroid differentiation and is repressed by a TAL1–PRMT6 complex

## TL;DR

This study shows that the TAL1–PRMT6 complex represses ID3, which is important for erythroid differentiation, and that increasing ID3 expression can boost erythrocyte production.

## Contribution

The study identifies a novel TAL1–PRMT6 complex that represses ID3 during erythropoiesis.

## Key findings

- TAL1 and PRMT6 interact at the ID3 promoter to repress its expression in progenitor cells.
- ID3 expression increases during erythroid differentiation as repression by TAL1–PRMT6 is relieved.
- Overexpression of ID3 enhances erythropoiesis in primary hCD34+ cells.

## Abstract

Erythropoiesis is controlled by transcription factors that recruit epigenetic cofactors to establish and maintain erythrocyte-specific gene expression patterns while repressing alternative lineage commitment. The transcription factor TAL1 (T-cell acute lymphocytic leukemia 1) is critical for establishing erythroid gene expression. It acts as an activator or repressor of genes, depending on associated epigenetic cofactors. Understanding the epigenetic function of TAL1 during erythropoiesis is key to improving in vitro erythroid differentiation and understanding pathological erythropoiesis. Therefore, the regulatory mechanisms that control the function of TAL1 during erythropoiesis are under intense investigation. Here, we show that TAL1 interacts with protein–arginine–methyltransferase-6 (PRMT6) on the ID3 (inhibitor-of-DNA-binding-3) gene in K562 and hCD34+ cells. The ID protein family is a critical transcriptional regulator of hematopoietic cell differentiation. We show that TAL1 and PRMT6 are present at the ID3 promoter, and that TAL1 is involved in the recruitment of PRMT6. Here, PRMT6 epigenetically regulates ID3 expression by mediating dimethylation of histone 3 at arginine 2. Thus, TAL1–PRMT6 epigenetically represses ID3 expression in progenitors, which is relieved upon erythroid differentiation, leading to increased expression. Overexpression of ID3 in primary hCD34+ cells enhances erythropoiesis. Our results show that a TAL1–PRMT6 complex regulates genes important for erythropoiesis, such as ID3. Manipulation of ID3 expression may be a way to promote in vitro differentiation of hCD34+ cells into erythrocytes.