# Ubiquitin-like and ubiquitinylated proteins associated with the maternal cell walls of Scenedesmus obliquus 633 as identified by immunochemistry and LC–MS/MS proteomics

## TL;DR

This study identifies ubiquitin-like and ubiquitinylated proteins in the maternal cell walls of the green alga Scenedesmus obliquus using immunochemistry and proteomics.

## Contribution

The study is the first to demonstrate the presence of ubiquitin-like proteins and ubiquitinated peptides in the maternal cell walls of Scenedesmus obliquus.

## Key findings

- Immunoblotting and immunofluorescence revealed ubiquitin-like epitopes in maternal cell walls.
- LC–MS/MS identified 95 proteins, including ubiquitin and ubiquitin-related proteins.
- Putative ubiquitin conjugates of higher molecular mass were detected in the cell walls.

## Abstract

The cell walls of green algae Scenedesmus obliquus are complex, polymeric structures including an inner cellulose layer surrounded by an algaenan-containing trilaminar sheath. The process of autosporulation leads to the formation of sporangial (maternal) cell walls, which are released into the medium after sporangial autolysis. In this study, a fraction of maternal cell wall material (CWM) was isolated from the stationary phase cultures of Scenedesmus obliquus 633 and subjected to immunofluorescence microscopy using polyclonal anti-ubiquitin antibodies. The water-extracted polypeptide fraction from the maternal cell walls was then analyzed using immunoblotting and LC–MS/MS. An immunoanalysis showed the presence of several peptides reactive with polyclonal anti-ubiquitin serum, with apparent molecular masses of c. 12, 70, 120, 200, and > 250 kDa. Cell wall–associated peptides were identified on the basis of LC–MS/MS spectra across NCBI databases, including the Scenedesmaceae family (58 records), the Chlorophyceae class (37 records), and Chlamydomonas reinhardtii (18 records) corresponding to the signatures of 95 identified proteins. In particular, three signatures identified ubiquitin and ubiquitin-related proteins. In the maternal cell walls, immunoblotting analysis, immunofluorescence microscopy, and LC–MS/MS proteomics collectively demonstrated the presence of ubiquitin-like epitopes, ubiquitin-specific peptide signatures, and several putative ubiquitin conjugates of a higher molecular mass. These results support the presence of ubiquitin-like proteins in the extramembranous compartment of Scenedesmus obliquus 633 and suggest that protein ubiquitination plays a significant role in the formation and functional integrity of the maternal cell walls in green algae.

The online version contains supplementary material available at 10.1007/s00709-024-01994-3.