# At5g63290 does not encode coproporphyrinogen III oxidase **Authors:** Wenjuan Ji, Huijuan Wang, Ning An, Xuan Zhou, Bingxiao Wen, Bernhard Grimm, Zhenhua Liu PMC · DOI: 10.1093/plphys/kiaf046 · Plant Physiology · 2025-01-29 ## TL;DR This paper shows that the gene At5g63290 in Arabidopsis does not perform a key step in tetrapyrrole production, changing the known pathway. ## Contribution The study corrects the assumed function of At5g63290 in the tetrapyrrole biosynthesis pathway. ## Key findings - At5g63290 does not catalyze the decarboxylation of coproporphyrinogen III. - The tetrapyrrole biosynthesis pathway in Arabidopsis needs to be revised. ## Abstract At5g63290 is not responsible for the decarboxylation of coproporphyrinogen III, prompting a revision of the tetrapyrrole biosynthesis pathway in Arabidopsis thaliana. ## Linked entities - **Genes:** AT5G63290 (Radical SAM superfamily protein) [NCBI Gene 836449] - **Chemicals:** coproporphyrinogen III (PubChem CID 321) - **Species:** Arabidopsis thaliana (taxon 3702) ## Full-text entities - **Genes:** AT5G63290 (Radical SAM superfamily protein) [NCBI Gene 836449] {aka MDC12.26, MDC12_26} - **Diseases:** sterility (MESH:D007246) - **Chemicals:** harderoporphyrinogen ( (MESH:C018563), CPO (-), Tetrapyrrole (MESH:D045725), 5-ALA (MESH:D000622), coproporphyrinogen III (MESH:C028026), S-adenosyl-l-methionine (MESH:D012436), chlorophyll (MESH:D002734), SDS (MESH:D012967), oxygen (MESH:D010100), dithionite (MESH:D004227), heme (MESH:D006418), protoporphyrinogen IX (MESH:C011539), carbon (MESH:D002244), phytochromobilin (MESH:C075811), Fe-S (MESH:D007501), sulfur (MESH:D013455), 5-aminolevulinic acid (MESH:C000614854), uroporphyrinogen (MESH:D014577), siroheme (MESH:C009272), Cys (MESH:D003545), 5-deoxyadenosine (MESH:C033714) - **Species:** Arabidopsis thaliana (mouse-ear cress, species) [taxon 3702], Nicotiana benthamiana (species) [taxon 4100], Saccharomyces cerevisiae (baker's yeast, species) [taxon 4932], Mus musculus (house mouse, species) [taxon 10090], Escherichia coli (E. coli, species) [taxon 562], Rattus norvegicus (brown rat, species) [taxon 10116], Lactococcus lactis (species) [taxon 1358] - **Cell lines:** S2 — Drosophila melanogaster (Fruit fly), Spontaneously immortalized cell line (CVCL_Z232), (DE3 — Mus musculus (Mouse), Hybridoma (CVCL_B7HM) ## Full text _Full body text omitted from this summary view._ Fetch the complete paper as Markdown: https://tomesphere.com/paper/PMC11834973/full.md ## Figures 2 figures with captions in the complete paper: https://tomesphere.com/paper/PMC11834973/full.md ## References 20 references — full list in the complete paper: https://tomesphere.com/paper/PMC11834973/full.md --- Source: https://tomesphere.com/paper/PMC11834973