# IL-6–174 G/C transversion might decrease male infertility risk: A case-control study

## TL;DR

This study found that a specific genetic variation in the IL-6 gene may lower the risk of male infertility, especially for certain subtypes like oligozoospermia and asthenospermia.

## Contribution

The study identifies a novel protective role of the IL-6–174 G/C polymorphism in reducing male infertility risk.

## Key findings

- The GC genotype of IL-6–174 G/C is significantly associated with male infertility.
- The C allele correlates with reduced risk of infertility and specific subtypes like oligozoospermia and asthenospermia.
- The heterozygous GC genotype is linked to a lower risk of oligozoospermia and asthenospermia.

## Abstract

Genetic predispositions have been identified as important factors in male infertility. Among the many genes related to male reproductive function, interleukin 6 (IL-6) has emerged as a key player. Despite the growing recognition of genetic factors in male infertility, the specific association between the IL-6–174 G/C genetic polymorphism and male infertility remains an area that needs further investigation.

This investigation explores the correlation between the IL-6–174 G/C transversion and male infertility.

In a case-control study, a total of 314 men who referred to the Kashan Infertility Center, Shahid Beheshti hospital, Kashan, Iran, were enrolled for IL-6–174 G/C polymorphism analysis. The study comprised 163 infertile participants as the case group and 151 fertile men as the control group. Following the screening, 2 ml of whole blood was collected from each participant. Cases were categorized into 3 subgroups based on World Health Organization criteria: (i) nonobstructive azoospermia (n = 42), (ii) oligozoospermia (n = 61), and (iii) asthenozoospermia (n = 60). After DNA extraction, genotypes of the samples at the -174 G/C (rs1800795) locus were determined using the polymerase chain reaction-restriction fragment length polymorphism method.

Our genetic investigation demonstrated a significant association between the GC genotype and male infertility. Furthermore, a correlation was observed between the heterozygous GC genotype and reduced risk of oligozoospermia and asthenospermia. Additionally, the C allele was correlated with a decreased risk of infertility and specific subgroups such as oligozoospermia and asthenospermia.

Our findings suggest that the IL-6–174 G/C transversion could potentially serve as a protective genetic factor against male infertility.