# Establishment of a Highly Efficient In Vitro Regeneration System for Nandina domestica ‘Firepower’

## TL;DR

This paper establishes an efficient in vitro regeneration system for Nandina domestica ‘Firepower’ to improve its propagation for industrial use.

## Contribution

The study introduces a novel in vitro regeneration system using direct organogenesis for Nandina domestica ‘Firepower’.

## Key findings

- The optimal medium for axillary shoot proliferation was 1/2 MS + 1.5 mg L−1 BA + 0.01 mg L−1 IBA with a multiplication rate of 9.22.
- Rooting was best achieved using 1/2 MS + 0.2 mg L−1 NAA + 0.3 mg L−1 IBA + 0.2 mg L−1 AC.
- A peat soil, vermiculite, and perlite (2:2:1) substrate was optimal for hardening and cultivation of plantlets.

## Abstract

Nandina domestica ‘Firepower’ is one of the most popular colorful foliage species in landscaping. However, it is currently propagated mainly by seeding and cuttings, with a low reproduction coefficient, hindering the cultivation of this species. Therefore, establishing an in vitro regeneration system would be beneficial for the industrialized production of Nandina domestica ‘Firepower’. In this study, an ex vivo regeneration system was established using the direct organogenesis pathway. In early April, the new shoots of Nandina domestica ‘Firepower’ were selected, and the stem segments of 1~2 cm were cut as the disinfection materials for the explants. The optimal formulation for inducing axillary shoots was 1/2 MS + 1.5 mg L−1 6-benzylaminopurine (BA) + 0.3 mg L−1 indole-3-butric acid (IBA). The optimal formulation for the differentiation and proliferation of axillary shoots was 1/2 MS + 1.5 mg L−1 BA + 0.01 mg L−1 IBA with a multiplicity of proliferation of 9.22. We determined that the rooting of axillary shoots required a combination of IBA, naphthalene acetic acid (NAA), and activated carbon (AC). The optimal formulation for rooting was 1/2 MS + 0.2 mg L−1 NAA + 0.3 mg L−1 IBA + 0.2 mg L−1 AC. After a two-day hardening period for tissue-cultured plantlets, a substrate consisting of peat soil, vermiculite, and perlite at a ratio of 2:2:1 was determined to be the optimal cultivation formulation. This system provides a framework for the industrialized production of Nandina domestica ‘Firepower’.