# Alterations in Implantation Genes and Dendritic Cells in Endometrial Samples After Antibiotic Treatment

## TL;DR

This study shows that antibiotic treatment for chronic endometritis improves implantation-related genes and clinical outcomes in some patients.

## Contribution

The novel finding is that antibiotic treatment reverses gene expression changes linked to implantation in endometrial tissue affected by chronic endometritis.

## Key findings

- Endometrial expression of HOXA10 and IGF-1 genes increased significantly after antibiotic treatment.
- Dendritic cell marker CD141 expression decreased following treatment.
- Six out of ten patients conceived and delivered after treatment.

## Abstract

Background/Objectives: This retrospective study assessed the impact of chronic endometritis (CE) on the expression of implantation genes HOXA10 (Homeobox A10) and IGF-1 (insulin-like growth factor 1), and on dendritic cells before and after antibiotic treatment, as well as on clinical reproductive outcomes. Methods: The study was conducted from 2021 to 2022. Ten assisted reproductive technology patients who underwent an endometrial biopsy before antibiotic treatment, confirming the diagnosis of CE, and a second biopsy after completing a course of 100 mg doxycycline twice daily for 14 days were included. Paraffin-embedded endometrial samples from these patients were obtained from the pathology department. The samples were evaluated for quantifying implantation genes HOXA10 and IGF-1 using RT-PCR, and for identification of dendritic cells using immunohistochemical staining of CD141. Conceptions and live births were also evaluated. Results: Endometrial expression of HOXA10 and IGF-1 genes was significantly elevated after antibiotic treatment and expression of dendritic cells CD141 was decreased. Eight of the ten patients required second-line antibiotic treatment due to persistent CE. Six patients conceived and delivered. Conclusions: CE is reversible with antibiotic treatment that resulted in improvements in implantation genes and clinical results. A high proportion of patients required broader spectrum antibiotic treatment.