Quantitative Proteomic Analysis of Lysine Malonylation in Response to Salicylic Acid in the Roots of Platycodon grandiflorus
Wanyue Ding, Yingying Duan, Yuqing Wang, Jizhou Fan, Weiyi Rao, Shihai Xing
TL;DR
This study explores how salicylic acid affects lysine malonylation in the roots of Platycodon grandiflorus, revealing its role in plant metabolism and stress response.
Contribution
The first comprehensive quantitative proteomic analysis of lysine malonylation in Platycodon grandiflorus roots after salicylic acid treatment.
Findings
Salicylic acid treatment altered lysine malonylation levels in 798 modification sites across 414 proteins.
Malonylation was primarily linked to photosynthesis and carbon metabolism pathways.
Salicylic acid increased malondialdehyde and enzyme activity but did not affect saponin content.
Abstract
Salicylic acid, as a plant hormone, significantly affects the physiological and biochemical indexes of soluble sugar, malondialdehyde content, peroxidase, and superoxide dismutase enzyme activity in Platycodon grandiflorus. Lysine malonylation is a post-translational modification that involves various cellular functions in plants, though it is rarely studied, especially in medicinal plants. In this study, the aim was to perform a comparative quantitative proteomic study of malonylation modification on P. grandiflorus root proteins after salicylic acid treatment using Western blot with specific antibodies, affinity enrichment and LC-MS/MS analysis methods. The analysis identified 1907 malonyl sites for 809 proteins, with 414 proteins and 798 modification sites quantified with high confidence. Post-treatment, 361 proteins were upregulated, and 310 were downregulated. Bioinformatics…
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Taxonomy
TopicsPlant Gene Expression Analysis · Ubiquitin and proteasome pathways · Tannin, Tannase and Anticancer Activities