# Hsa_circ_0000105 promotes nasopharyngeal carcinoma malignancy by miR-541-3p/S100A11 axis

## TL;DR

This study shows that hsa_circ_0000105 promotes the spread of nasopharyngeal cancer by interacting with miR-541-3p and S100A11.

## Contribution

The study identifies a novel regulatory axis involving hsa_circ_0000105, miR-541-3p, and S100A11 in nasopharyngeal carcinoma.

## Key findings

- Hsa_circ_0000105 is highly expressed in NPC and promotes cancer cell malignancy.
- Hsa_circ_0000105 competitively binds miR-541-3p, leading to increased S100A11 expression.
- Modulating hsa_circ_0000105 affects tumor growth in vivo.

## Abstract

•hsa_circ_0000105 modulates malignancy of NPC cells.•hsa_circ_0000105 influences the malignant behavior of NPC via downstream miR-541-3p.•hsa_circ_0000105 positively regulates S100A11 to accelerate the malignant behavior of NPC.•hsa_circ_0000105 inhibits NPC tumor growth in vivo.

hsa_circ_0000105 modulates malignancy of NPC cells.

hsa_circ_0000105 influences the malignant behavior of NPC via downstream miR-541-3p.

hsa_circ_0000105 positively regulates S100A11 to accelerate the malignant behavior of NPC.

hsa_circ_0000105 inhibits NPC tumor growth in vivo.

This study was to investigate whether hsa_circ_0000105 is involved in the process of regulating Nasopharyngeal Carcinoma (NPC) biological behaviors and to reveal the molecular mechanism.

NPC tissues and normal tissues were collected, and NPC cell lines and normal control cell lines were obtained. hsa_circ_0000105/miR-541-3p/S100A11 was evaluated by RT-qPCR or Western blot. CCK-8 assay, EdU assay, Transwell assay, wound healing assay, flow cytometry, and Western blot were employed to evaluate the biological behaviors of NPC cells. Dual luciferase reporter assay and RIP assay were applied to evaluate the direct targeting relationship of hsa_circ_0000105/miR-541-3p/S100A11. A tumor xenotransplantation assay was implemented to evaluate the effect of hsa_circ_0000105 on NPC tumors.

hsa_circ_0000105 and S100A11 were highly expressed in NPC, while miR-541-3p was lowly expressed. hsa_circ_0000105 competitively adsorbed miR-541-3p and mediated S100A11 expression. Silencing or upregulation of hsa_circ_0000105 restricts and induces malignant behavior in HNE2 cells, respectively. The impact of hsa_circ_0000105 silencing on malignant behaviors of HNE2 cells was blocked by miR-541-3p downregulation, while that of hsa_circ_0000105 upregulation was attenuated by S100A11 inhibition.

hsa_circ_0000105 acts as a carcinogenic factor in NPC and promotes NPC malignancy by miR-541-3p/S100A11 axis.