Genome sequence of Leptolyngbya phage Dor1, a cyanophage induced from a fish pond

TL;DR

Scientists sequenced a new cyanophage, Dor1, from a fish pond and found it shares most of its genome with another phage but has a unique DNA-cutting enzyme.

Contribution

The discovery of an HNH endonuclease in the DNA polymerase gene of Dor1 adds a novel feature to cyanophage genomic diversity.

Findings

Dor1's genome is 41,522 bp long and shares 93.77% similarity with LPP-1.

Dor1 uniquely contains an HNH endonuclease in its DNA polymerase gene.

Dor1 was isolated from Leptolyngbya boryana using mitomycin C induction.

Abstract

Leptolyngbya phage Dor1 was induced by mitomycin C from a fishpond and was isolated on Leptolyngbya boryana IU 594. The 41,522-bp genome of Leptolyngbya phage Dor1 has 93.77% intergenomic similarity with Leptolyngbya phage LPP-1; however, unlike LPP-1, Dor1 carries an HNH endonuclease in its DNA polymerase gene.