# Comparison of anesthetic effects of xylazine combined with alfaxalone or ketamine and maintained with isoflurane in captive Formosan Reeve’s Muntjac (Muntiacus reevesi micrurus)

## TL;DR

This study compares two anesthetic combinations for Formosan Reeve’s muntjacs, finding that both work well but one causes more breathing issues.

## Contribution

The study provides new empirical data on safe and effective anesthetic protocols for a specific deer species with limited prior research.

## Key findings

- Ketamine-xylazine (KX) and alfaxalone-xylazine (AX) both induce anesthesia effectively in muntjacs.
- AX causes less severe hypoxemia compared to KX, with better oxygen saturation levels observed.
- Recovery times are shorter with AX compared to KX, though both are considered satisfactory.

## Abstract

Formosan Reeve’s muntjac is a Cervidae species endemic to Southeast China and Taiwan. However, research on different anesthetic protocols, their effects, and their safety in Formosan Reeve’s muntjac is limited. This study evaluated the effects of ketamine-xylazine (KX) and alfaxalone-xylazine (AX) administered via blow darts to nine muntjacs. Induction and recovery times as well as the quality were assessed by a blinded observer. Peripheral oxygen saturation (SpO2), heart rate, respiratory rate, and rectal temperature were recorded for at least 30 min. Tolazoline (4 mg/kg) was used post-procedure to reverse xylazine’s effects. The mean doses were 4.68 ± 2.18 mg/kg for ketamine and 3.22 ± 1.33 mg/kg for xylazine in the KX group. In the AX group, the mean doses were 4.38 ± 0.31 mg/kg for alfaxalone and 1.19 ± 0.26 mg/kg for xylazine. The median induction times were 339.5 s (range 180.0-375.0) for KX and 125.0 s (range 71.0–334.0) for AX, with both groups scoring 3.0 for induction quality. The recovery times were 507.5 s (range 266.0–1081.0) for KX and 243.0 s (range 92.0–480.0) for AX, with recovery scores of 2.3 and 3.0, respectively, showing no significant difference. Hypoxemia (SpO2 < 90%) was more severe in the KX group when compared to the AX group (SpO2 > 92%), and rectal temperatures were higher in the former during the first 15 min. Heart and respiratory rates showed no significant differences between groups. Our findings demonstrate that both anesthetic combinations achieve reliable induction and satisfactory recovery in Formosan Reeve’s muntjac, with the ketamine-xylazine combination causing a more profound hypoxemia post-induction compared to the alfaxalone-xylazine combination.