# RNA helicase MOV10 suppresses fear memory and dendritic arborization and regulates microtubule dynamics in hippocampal neurons

## TL;DR

The RNA helicase MOV10 regulates fear memory and dendritic growth in neurons by controlling the translation of NUMA1, a protein involved in microtubule dynamics.

## Contribution

This study is the first to show that MOV10 regulates NUMA1 translation, linking it to dendritic arborization and fear memory.

## Key findings

- Brain-specific MOV10 knockout mice show enhanced fear memory and elongated dendrites.
- NUMA1 mRNA is a MOV10 target, and its reduced expression in MOV10 knockout neurons is rescued by restoring NUMA1 or knocking down HAUS.
- MOV10's role in regulating microtubule dynamics via NUMA1 provides a new mechanism for dendritogenesis.

## Abstract

RNA helicase MOV10 is highly expressed in postnatal brain and associates with FMRP and AGO2, suggesting a role in translation regulation in learning and memory.

We generated a brain-specific knockout mouse (Mov10 Deletion) with greatly reduced MOV10 expression in cortex and hippocampus. Behavior testing revealed enhanced fear memory, similar to that observed in a mouse with reduced brain microRNA production, supporting MOV10’s reported role as an AGO2 cofactor. Cultured hippocampal neurons have elongated distal dendrites, a reported feature of augmin/HAUS over-expression in Drosophila da sensory neurons. In mitotic spindle formation, HAUS is antagonized by the microtubule bundling protein NUMA1. Numa1 mRNA is a MOV10 CLIP target and is among the genes significantly decreased in Mov10 Deletion hippocampus. Restoration of NUMA1 expression and knockdown of HAUS rescued phenotypes of the Mov10 Deletion hippocampal neurons.

This is the first evidence of translation regulation of NUMA1 by MOV10 as a control point in dendritogenesis.

The online version contains supplementary material available at 10.1186/s12915-025-02138-6.