# Dafadine Does Not Promote Dauer Development in Pristionchus pacificus

## TL;DR

This study finds that Dafadine-A does not trigger dauer development in Pristionchus pacificus, unlike in other nematodes.

## Contribution

The paper reveals that Dafadine-A's effect on dauer development is not conserved in Pristionchus pacificus.

## Key findings

- Dafadine-A does not cause dauer formation in Pristionchus pacificus.
- Dafadine-A may slightly reduce the growth rate of Pristionchus pacificus.
- The role of DAF-9 in dafachronic acid biosynthesis may differ in Pristionchus pacificus.

## Abstract

In response to unfavorable conditions, nematodes develop into the stress-resistant dauer larvae. Under favorable conditions, many nematodes are known to synthesize dafachronic acids (DAs) that bind to the conserved nuclear hormone receptor
DAF-12
to suppress dauer development. However, the enzymes involved in the production of DAs have not been thoroughly investigated in

Pristionchus pacificus

. Here we show that the cytochrome P450 inhibitor Dafadine-A, which suppresses
DAF-9
in DA biosynthesis in

C. elegans

and other nematode species, does not cause constitutive dauer formation or gonad migration defects in
P. pacificus
wild type. Instead, Dafadine-A may slightly reduce
P. pacificus
growth rate.