# Genetic Characterization of Myf5 and POU1F1 Genes in Different Egyptian Local Rabbit Breeds and Their Association with Growth Traits

## TL;DR

This study examines genetic variations in the MYF5 and POU1F1 genes among Egyptian rabbit breeds and links them to growth traits.

## Contribution

The study identifies specific genotypes in MYF5 and POU1F1 genes associated with growth traits in Egyptian rabbit breeds.

## Key findings

- MYF5 exon 1 showed two genotypes in BB, GB, and NZW breeds but only one in APRI.
- Genotypes of Myf5 exon 2 and POU1F1 were linked to growth weight differences in rabbit breeds.
- Certain genotypes in MYF5 and POU1F1 are proposed as molecular markers for improving growth traits.

## Abstract

Genetic characterization and its association with quantitative traits in local breeds are important tools for the genetic improvement and sustainable management of animal genetic resources. Myogenic regulatory factor 5 (MYf5) and POU class 1 homeobox 1 (POU1F1) are candidate genes which play important roles in growth and development of mammals. The present study aims to detect the genetic diversity of the MYf5 and POU1F1 genes in four local Egyptian rabbit breeds and their association with growth traits, using PCR-restriction enzyme (PCR–RFLP), PCR-single-strand conformational polymorphism (PCR–SSCP), and direct sequencing techniques. The results showed that MYF5 exon 1 was observed with two genotypes in Baladi Black (BB), Gabali (GB) and New Zealand White (NZW) breeds while APRI-line (APRI) presented one genotype. The genetic diversity of Myf5 exon 2 between breeds showed two genotypes in APRI compared to three in NZW and four genotypes in BB and GB breeds. The genetic diversity of the POU1F1 gene (intron 5 and partial cds) in different rabbit breeds was two genotypes in NZW and three genotypes in BB, GB, and APRI breeds with different frequencies for each genotype. Based on the statistically significant difference between genes genotypes and growth weight, the results suggested that the genotypes of Myf5 exon 2 (1 and 2) of the BB breed, Myf5 exon 2 genotype 2 of the APRI breed, and genotype 1 of Myf5 exon 1 and genotype 1 of POU1F1 of the NZW breed compared to genotypes for each gene can be considered candidate molecular markers associated with the improvement of growth traits in these breeds.