# Antibiotic-induced dysbiosis in the SCIME™ recapitulates microbial community diversity and metabolites modulation of in vivo disease

## TL;DR

A new in vitro model simulates antibiotic-induced gut dysbiosis in dogs and shows how prebiotics may help recovery.

## Contribution

A novel in vitro model of canine antibiotic-induced dysbiosis that mimics in vivo microbial and metabolic changes.

## Key findings

- Antibiotic treatment in the model caused decreased microbial richness and propionic acid production.
- Dietary changes altered ammonium and butyric acid levels in the simulated gut ecosystem.
- The model successfully recapitulates microbial and metabolic patterns of in vivo canine dysbiosis.

## Abstract

Intestinal dysbiosis is a significant concern among dog owners, and the gut health of pets is an emerging research field. In this context, the Simulator of the Canine Intestinal Microbial Ecosystem (SCIME™) was recently developed and validated with in vivo data.

The current study presents a further application of this model by using amoxicillin and clavulanic acid to induce dysbiosis, aiming to provoke changes in microbial community and metabolite production, which are well-known markers of the disease in vivo.

Following the induction of dysbiosis, prebiotic supplementation was tested to investigate the potential for microbiota recovery under different dietary conditions.

The results showed that antibiotic stimulation in the SCIME™ model can produce significant changes in microbial communities and metabolic activity, including a decrease in microbial richness, a reduction in propionic acid production, and alterations in microbial composition. Additionally, changes in ammonium and butyric acid levels induced by the tested diets were observed.

This alteration in microbial community and metabolites production mimicks in vivo canine dysbiosis patterns. A novel dynamic in vitro model simulating canine antibiotic-induced dysbiosis, capable of reproducing microbial and metabolic changes observed in vivo, has been developed and is suitable for testing the effects of nutritional changes.