EV71 infection alters the lipid composition of human rhabdomyosarcoma (RD) cells-derived extracellular vesicles

TL;DR

This study shows that EV71 infection changes the lipid content of extracellular vesicles from RD cells, which could influence how these vesicles function in other cells.

Contribution

The study provides a detailed lipidomic analysis of EVs from EV71-infected RD cells, revealing specific lipid alterations.

Findings

EV71 infection significantly increased the lipid content of extracellular vesicles.

1705 lipid molecules across 43 lipid classes were identified in the EVs.

Lipidomic analysis revealed specific lipid molecules with significant differences between infected and uninfected EVs.

Abstract

Previous studies demonstrated that EV71-infected cells secrete extracellular vesicles (EVs), facilitating the transfer of viral components to recipient cells and thereby promoting virus spread. Considering lipid signaling plays a crucial role in EVs-mediated cell-to-cell communication, we compared the lipid profile of EVs secreted from uninfected and EV71-infected cells (EVs-Mock and EVs-EV71) using the human rhabdomyosarcoma (RD) cell model. These two groups of EVs were purified by using size exclusion chromatography (SEC), respectively, and evaluated by transmission electron microscopy (TEM), nanoparticle tracking technology (NTA), and Western blotting (WB). In-depth lipidomic analysis of EVs identified 1705 lipid molecules belonging to 43 lipid classes. The data showed a significant increase in the lipid content of EVs after EV71 infection. Meanwhile, we deeply analyzed the changes in lipids and screened for lipid molecules with significant differences compared EVs-EV71 with EVs-Mock EVs. Altogether, we report the alterations in the lipid profile of EVs derived from RD-cells after EV71 infection, which may affect the function of the EVs in the recipient cells.

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