# Physiological and Transcriptomic Analyses Demonstrate the Ca2+-Mediated Alleviation of Salt Stress in Magnolia wufengensis

## TL;DR

This study shows how calcium helps Magnolia wufengensis plants resist salt stress by improving growth and activating specific genes.

## Contribution

The study identifies key genes and pathways through which Ca2+ mitigates salt stress in M. wufengensis.

## Key findings

- Combined NaCl and CaCl2 treatment reduced salt-induced damage and restored plant growth.
- RNA sequencing identified 968 and 2632 differentially expressed genes in response to salt and Ca2+ treatments.
- Key genes related to transport, signaling, and antioxidant enzymes were highlighted as important for salt tolerance.

## Abstract

Magnolia wufengensis, a newly discovered ornamental species in the Magnoliaceae family, is susceptible to salinity. Moreover, Ca2+ is an essential element for plant growth and is receiving increasing attention for its ability to mitigate the negative effects of environmental stress on plants. In the present study, we investigated the effect of Ca2+ on the growth and transcriptome of M. wufengensis under salt stress. The treatments used here were as follows: control, NaCl (150 mmol/L), CaCl2 (5 mmol/L), and NaCl (150 mmol/L) + CaCl2 (5 mmol/L). After a 60-day treatment period, plant growth indices were determined, and leaves were collected for physiological analysis and transcriptome investigation. The combined application of NaCl and CaCl2 alleviated phenotypic damage and restored seedling growth. Moreover, RNA sequencing data revealed that in the Na vs. control group and the NaCa vs. Na group, there were 968 and 2632 differentially expressed genes, respectively, which were both primarily enriched in secondary metabolism, glutathione metabolism, signaling hormone metabolism, glucose metabolism, and amino acid metabolism. These pathways were analyzed to screen key genes: the adenosine triphosphate (ATP)-binding cassette efflux transporter G1 (ABCG1) genes, which are related to transmembrane transport; the calmodulin genes, which are related to signal transmission; and the glutathione S-transferase (GST), glutathione peroxidase (GPX), and peroxidase (POD) genes related to antioxidant enzymes. Lastly, we constructed a hypothesis model of Ca2+-enhanced salt tolerance in M. wufengensis. This study reveals the potential mechanisms by which Ca2+ enhances the salt tolerance of M. wufengensis and provides a theoretical reference for its cultivation in saline areas.