# Establishment of a Rapid Detection Method for Cadmium Ions via a Specific Cadmium Chelator N-(2-Acetamido)-Iminodiacetic Acid Screened by a Novel Biological Method

## TL;DR

This study introduces a fast, safe, and low-cost method to detect cadmium in agricultural products using a new chelator and colorimetric technique.

## Contribution

A novel biological method was used to screen a specific cadmium chelator for rapid detection in agricultural products.

## Key findings

- The method has a detection limit of 0.08 μM and a linear range of 0.1–10 μM for cadmium.
- The paper strip method allows naked-eye detection in under 1 minute with a limit of 0.2 μM.
- Results matched well with ICP-MS for cadmium in rice, soybean, milk, and other samples.

## Abstract

Heavy metal ions such as cadmium, mercury, lead, and arsenic in the soil cannot be degraded naturally and are absorbed by crops, leading to accumulation in agricultural products, which poses a serious threat to human health. Therefore, establishing a rapid and efficient method for detecting heavy metal ions in agricultural products is of great significance to ensuring the health and safety. In this study, a novel optimized spectrometric method was developed for the rapid and specific colorimetric detection of cadmium ions based on N-(2-Acetamido)-iminodiacetic acid (ADA) and Victoria blue B (VBB) as the chromogenic unit. The safety evaluation of ADA showed extremely low biological toxicity in cultured cells and live animals. The standard curve is y = 0.0212x + 0.1723, R2 = 0.9978, and LOD = 0.08 μM (0.018 mg/kg). The liner concentrations detection range of cadmium is 0.1–10 μM. An inexpensive paper strip detection method was developed with a detection limit of 0.2 μM to the naked eye and a detection time of less than 1 min. The method was successfully used to assess the cadmium content of rice, soybean, milk, grape, peach, and cabbage, and the results correlated well with those determined by inductively coupled plasma–mass spectrometry (ICP-MS). Thus, our study demonstrated a novel rapid, safe, and economical method for onsite, real-time detection of cadmium ions in agricultural products.