Low Immunogenicity of Keratinocytes Derived from Human Embryonic Stem Cells

TL;DR

This study shows that keratinocytes from human embryonic stem cells have low immunogenicity, making them promising for regenerative medicine.

Contribution

The study demonstrates the low immunogenicity of embryonic stem cell-derived keratinocytes in both in vitro and in vivo models.

Findings

ESKCs exhibit low HLA molecule expression and limited antigen presentation capabilities.

ESKCs weakly stimulate inflammatory responses in allogeneic PBMCs and show minimal rejection in humanized mice.

Abstract

Epidermal transplantation is a common and widely used surgical technique in clinical medicine. Derivatives of embryonic stem cells have the potential to serve as a source of transplantable cells. However, allograft rejection is one of the main challenges. To investigate the immunogenicity of keratinocytes derived from human embryonic stem cells (ESKCs), we conducted a series of in vivo and in vitro experiments. The results showed that ESKCs have low HLA molecule expression, limited antigen presentation capabilities, and a weak ability to stimulate the proliferation and secretion of inflammatory factors in allogeneic PBMCs in vitro. In humanized immune mouse models, ESKCs elicited weak transplant rejection responses in the host. Overall, we found that ESKCs have low immunogenicity and may have potential applications in the field of regenerative medicine.

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