# Comparative proteomics analysis of the mouse mini-gut organoid: insights into markers of gluten challenge from celiac disease intestinal biopsies

## TL;DR

This study uses mouse mini-gut organoids to identify proteins affected by gluten challenge, offering insights into celiac disease markers.

## Contribution

The study introduces a mouse mini-gut organoid model to identify gluten-induced proteomic changes relevant to celiac disease.

## Key findings

- LC–MS/MS identified 4,850 protein groups in mouse mini-gut organoids.
- 400 putative biomarkers showed differential expression after gluten challenge.
- Gluten challenge disrupted the crypt–villus axis in the organoid model.

## Abstract

Organoid models enable three-dimensional representation of cellular systems, providing flexible and accessible research tools, and can highlight key biomolecules. Such models of the intestinal epithelium can provide significant knowledge for the study of celiac disease and provide an additional context for the nature of markers observed from patient biopsy data.

Using LC–MS/MS, the proteomes of the crypt and enterocyte-like states of a mouse mini-gut organoid model were measured. The data were further compared with published biopsy data by comparing the changes induced by gluten challenge after a gluten-free diet.

These analyses identified 4,850 protein groups and revealed how 400 putative biomarkers of dietary challenge were differentially expressed in the organoid model. In addition to the extensive changes within the differentiated cells, the data reiterated the disruption of the crypt–villus axis after gluten challenge. The mass spectrometry data are available via ProteomeXchange with the identifier PXD025690.