# Effect of Smoking on Seminal Plasma Vitamin B6 in Fertile and Subfertile Men

## TL;DR

This study found that smoking does not significantly affect vitamin B6 levels in the seminal plasma of fertile and subfertile men.

## Contribution

The study is the first to investigate the direct effect of smoking on seminal plasma vitamin B6 concentrations in both fertile and subfertile men.

## Key findings

- Smoking had no significant effect on seminal plasma vitamin B6 levels in fertile men.
- There was also no significant effect of smoking on vitamin B6 levels in subfertile men.
- No correlations were found between vitamin B6 levels, smoking duration, or age in any group.

## Abstract

Despite extensive documentation of the negative consequences of smoking on a wide range of diseases and disorders, the direct effect of smoking on seminal plasma vitamin B6 (vitB6) concentration is not addressed yet. Here, we attempted to examine the influence of smoking on the levels of seminal plasma vitB6 in fertile and subfertile males. One hundred and ninety-five participants were categorized into two groups: fertile men (smokers (n = 32), nonsmokers (n = 43)) and subfertile men (smokers (n = 65), nonsmokers (n = 55)). According to the World Health Organization criteria, conventional semen analysis was performed for all subjects involved in the study. The concentration of vitB6 in semen samples was measured by high-performance liquid chromatography tandem-mass spectrometry. There was no significant difference in the mean seminal plasma concentrations of vitB6 in smokers versus nonsmokers in both fertile (p=0.5096) and subfertile (p=0.5224) groups. Besides, no significant correlations were observed between seminal plasma concentration of vitB6, duration of smoking, and men's age in all tested groups. In conclusion, these findings elucidate that smoking has no significant impact on the levels of seminal plasma vitB6 in both fertile and subfertile men.