# Metagenomes, metagenome-assembled genomes, and metatranscriptomes from a chlorinated ethene-dechlorinating culture amended with biochar pyrolyzed at different temperatures

## TL;DR

This study explores how biochar and its pyrolysis temperature affect a microbial community that breaks down chlorinated ethene.

## Contribution

The novel contribution is the detailed metagenomic and metatranscriptomic analysis of a dechlorinating microbial consortium interacting with biochar.

## Key findings

- Biochar amendment altered microbial community structure and function.
- Pyrolysis temperature influenced microbial activity and gene expression.
- Metagenome-assembled genomes revealed insights into dehalogenating pathways.

## Abstract

We investigated the effects of biochar and pyrolysis temperature on a chlorinated ethene-dechlorinating anaerobic consortium. Sequencing of nucleic acids from suspended and biochar-attached cells yielded 9 metagenomes, 122 metagenome-assembled genomes, and 18 metatranscriptomes that provide insights into the structure, function, activity, and interactions of the dehalogenating consortium with biochar.