# A novel technique for atraumatic transurethral catheterisation of male rats

## TL;DR

Researchers developed a non-invasive method to catheterize male rats using a microscope and microsurgical tools, making it easier to study their urinary system.

## Contribution

A novel, atraumatic transurethral catheterisation method for male rats using microsurgical tools and a microscope.

## Key findings

- Six Wistar rats were successfully catheterised with no injury to the bladder or urethra.
- The technique allows for the inclusion of male rats in studies requiring urinary analysis or bladder irrigation.
- The method is straightforward to learn and less traumatic than percutaneous bladder puncture.

## Abstract

Transurethral catheterisation of male rats is technically difficult owing to anatomical peculiarities. In the male rat, the urethral striated sphincter consists of two lateral fascicles separated by an anterior and a posterior strip of connective tissue, which impedes the smooth insertion of a urinary catheter. For rat studies requiring continuous collection of urine, bladder irrigation, or measurement of bladder pressure, investigators either have to exclude the male population (be limited to the female population) or perform percutaneous (suprapubic) bladder puncture in male rats, which is more traumatic and invasive than transurethral catheterisation. This paper describes a novel, atraumatic method of transurethral catheterisation in the male rat, with the aid of a microscope and microsurgical instruments. Six Wistar rats were used for this experiment, all of which were catheterised successfully, with no evidence of bladder or urethral injury. The study shows that male rats can be safely catheterised via the urethra with the aid of a microscope and microsurgical instruments for both visual and tactile feedback. This is a relatively straightforward technique to learn and can allow for inclusion of male rats in future studies requiring urinary analysis or bladder irrigation, without the need for traumatic percutaneous (suprapubic) bladder puncture.

Summary: The study shows that male rats can be safely catheterised via the urethra with the aid of a microscope and microsurgical instruments for both visual and tactile feedback.