# Behavioural Responses of Tropical Bed Bug Cimex hemipterus (F.) (Hemiptera: Cimicidae) to Coloured Harbourage

## TL;DR

This study explores how tropical bed bugs choose colored shelters, finding they prefer red and black over white, which could help in developing better control methods.

## Contribution

The study reveals specific color preferences of tropical bed bugs for shelter and oviposition, offering insights for improved pest control strategies.

## Key findings

- Tropical bed bugs strongly prefer red and black colored harbourages over white ones.
- Female bed bugs prefer laying eggs on red-colored harbourages compared to black ones.
- Red-colored harbourages resulted in the highest population size of tropical bed bugs during rearing experiments.

## Abstract

Population of the tropical bed bug Cimex hemipterus (F.) (Hemiptera: Cimicidae), a temporary ectoparasite on both humans and animals, have surged in many tropical countries. Tropical bed bugs preferences when selecting a suitable harbourage and oviposition site were investigated. Two-choice and three choice colour assays were conducted to determine whether bed bugs will choose black, red or white coloured harbourages. Then, 50 1st instar were reared in containers containing black, red and white (control) paper served as the harbourages and observed for 12 weeks. Both fed and starve male, female and nymph strongly preferred red and black coloured harbourage compared to white coloured harbourage. Oviposition assays showed that female bed bugs prefered to laid their eggs on red coloured harbourages compared to black coloured harbourages. Rearing experiment showed that there was no significant difference (p > 0.05) between final population size of tropical bed bug. However, tropical bed bugs reared in container with red paper (600 ± 89.238) have the highest number of individuals followed by black (473 ± 133.841) and white (485 ± 84.234) paper. Bed bug preference towards coloured harbourage provide useful information for those developing new bed bug control method or improving bed bug infestation monitoring devices.