# Cleavage and polyadenylation factors are potential regulators of adipogenesis

## TL;DR

This study explores how cleavage and polyadenylation factors may influence fat cell development, finding changes in protein levels during differentiation.

## Contribution

The study identifies changes in cleavage and polyadenylation protein levels during adipogenesis, despite stable RNA levels.

## Key findings

- Protein levels of some cleavage and polyadenylation complex subunits change during 3T3-L1 differentiation.
- CPSF73 levels decrease during differentiation, but its overexpression does not alter differentiation efficiency.
- RNA expression of cleavage and polyadenylation proteins remains unchanged during adipogenesis.

## Abstract

Alternative polyadenylation (APA) is a co-transcriptional process that leads to isoform diversity in the 3’ ends of mRNAs. APA is known to occur during differentiation, and its dysregulation is observed in diseases like cancer and autoimmune disorders. It has been previously reported that differentiation of 3T3-L1 cells to adipocytes leads to an overall lengthening of mRNAs, but the proteins involved in this regulation have not been identified. The expression levels of subunits of the cleavage and polyadenylation (C/P) complex can regulate the choice of poly(A) site, which in turn can affect different cellular activities. In this paper, we studied the change in levels of C/P proteins during 3T3-L1 differentiation.

We observed that while the RNA expression of these proteins is unchanged during differentiation, the protein levels of some subunits do change, including a decrease in levels of CPSF73, the nuclease that cuts at the poly(A) site. However, overexpression of CPSF73 alone does not affect the efficiency and rate of differentiation.

The online version contains supplementary material available at 10.1186/s13104-024-06908-3.