# Evaluation of Surface Topography and Biomimetic Remineralization Capacity of Dendrimers in Comparison With Calcium Silicate Cement: An In Vitro Study

## TL;DR

This study shows that combining PAMAM dendrimers with Biodentine significantly improves root dentin remineralization compared to using either material alone.

## Contribution

The novel contribution is demonstrating that combining PAMAM dendrimers with Biodentine enhances remineralization of root dentin in an acid-challenging environment.

## Key findings

- Combining PAMAM and Biodentine led to the most significant root dentin remineralization.
- The hardness of demineralized root dentin reached levels similar to healthy dentin with the combination treatment.
- PAMAM + Biodentine showed superior performance in promoting remineralization compared to either material alone.

## Abstract

Introduction: Biodentine, a calcium silicate-based material, is known for its biocompatibility and ability to promote dentin regeneration. With their unique branching structure, polyamidoamine (PAMAM) dendrimers have shown promise in facilitating biomimetic remineralization processes.

Aim: This study investigates the synergistic effects of combining PAMAM with Biodentine on root dentin remineralization, aiming to develop a novel bioactive compound that offers superior protective and regenerative properties.

Methods: The following predictions were made: (1) In a cyclic artificial saliva/acid regimen, among the test groups, the combination of Biodentine and PAMAM would cause the most root dentin remineralization (2). Biodentine alone would increase Ca and P concentrations, neutralize acid, and promote root dentin remineralization (3). PAMAM, on the other hand, can remineralize the demineralized root dentin.

Results: Minimal mineral regeneration was accomplished in demineralized root dentin when treated with Biodentine or PAMAM alone. Root dentin remineralization was most pronounced when Biodentine and PAMAM were used together, and the hardness of demineralized root dentin was raised to an equivalent level to that of healthy root dentin.

Discussion: The study demonstrated the exceptional ability of PAMAM + Biodentine to promote root dentin remineralization. In an acid-challenging environment, PAMAM + Biodentine promoted full and efficient root dentin remineralization. Restorations made using innovative PAMAM + Biodentine technology show promise in remineralizing and protecting tooth structures.