# Generation of a highly specific and potent antibody against Ce-lamin/LMN-1

**Authors:** Adam Zahand, Byron Williams, Jun Liu

PMC · DOI: 10.17912/micropub.biology.001294 · microPublication Biology · 2024-08-15

## TL;DR

Researchers created a highly specific antibody against Ce-lamin/LMN-1, which helps study its stability during development.

## Contribution

A new highly specific and potent antibody against Ce-lamin/LMN-1 was generated and validated.

## Key findings

- The antibody detected maternally loaded Ce-lamin/LMN-1 protein through embryonic and larval stages.
- The protein remains detectable in homozygous null lmn-1 mutant adults.

## Abstract

High quality antibodies are useful tools for research in cell and developmental biology. We have obtained highly specific and potent guinea pig-derived polyclonal antibodies against Ce-lamin/
LMN-1
. Western blotting experiments using these antibodies demonstrated that maternally loaded Ce-lamin/
LMN-1
protein is very stable, can perdure through embryonic and larval development and remain detectable in homozygous null

lmn-1

mutant adults.

## Linked entities

- **Genes:** LMNA (lamin A/C) [NCBI Gene 4000]

## Full-text entities

- **Genes:** LMNA (lamin A/C) [NCBI Gene 4000] {aka CDCD1, CDDC, CMD1A, CMT2B1, EMD2, FPL}
- **Species:** Cavia porcellus (domestic guinea pig, species) [taxon 10141]

## Full text

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## Figures

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## References

11 references — full list in the complete paper: https://tomesphere.com/paper/PMC11362844/full.md

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Source: https://tomesphere.com/paper/PMC11362844