# A guide to selecting high-performing antibodies for Protein-glutamine gamma-glutamyltransferase 2 (TGM2) for use in western blot, immunoprecipitation and immunofluorescence

## TL;DR

This paper evaluates commercial antibodies for TGM2 to help researchers choose reliable ones for common lab techniques.

## Contribution

The study provides a standardized evaluation of TGM2 antibodies for western blot, immunoprecipitation, and immunofluorescence.

## Key findings

- Seventeen TGM2 antibodies were tested for western blot and sixteen for immunoprecipitation and immunofluorescence.
- A standardized protocol using knockout cell lines was used to assess antibody performance.
- The results are shared openly to improve antibody reproducibility and guide researchers.

## Abstract

Protein-glutamine gamma-glutamyltransferase 2 (TGM2) is a Ca
2+ dependent enzyme that catalyzes transglutaminase cross-linking modifications. TGM2 is involved in various diseases, either in a protective or contributory manner, making it a crucial protein to study and determine its therapeutic potential. Identifying high-performing TGM2 antibodies would facilitate these investigations. Here we have characterized seventeen TGM2 commercial antibodies for western blot and sixteen for immunoprecipitation, and immunofluorescence. The implemented standardized experimental protocol is based on comparing read-outs in knockout cell lines against their isogenic parental controls. This study is part of a larger, collaborative initiative seeking to address antibody reproducibility issues by characterizing commercially available antibodies for human proteins and publishing the results openly as a resource for the scientific community. While the use of antibodies and protocols vary between laboratories, we encourage readers to use this report as a guide to select the most appropriate antibodies for their specific needs.